Associations between dietary patterns and gene expression profiles of healthy men and women: a cross-sectional study.

Background: Diet regulates gene expression profiles by several mechanisms. The objective of this study was to examine gene expression in relation with dietary patterns.Methods: Two hundred and fifty four participants from the greater Quebec City metropolitan area were recruited. Two hundred and ten...

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Publicado en:Nutrition Journal Vol. 12; pp. 24 - 25
Autores principales: Bouchard-Mercier, Annie, Paradis, Ann-Marie, Rudkowska, Iwona, Lemieux, Simone, Couture, Patrick, Vohl, Marie-Claude
Formato: research Journal Article
Publicado: BioMed Central 2013
Acceso en línea:Ver este registro en EBSCOhost
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      pub: BioMed Central
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        10.1186/1475-2891-12-24
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        atl: Associations between dietary patterns and gene expression profiles of healthy men and women: a cross-sectional study.
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        au:
          Bouchard-Mercier, Annie
          Paradis, Ann-Marie
          Rudkowska, Iwona
          Lemieux, Simone
          Couture, Patrick
          Vohl, Marie-Claude
        affil: Institute of Nutraceuticals and Functional Foods (INAF), Laval University, 2440 Hochelaga Blvd, Quebec G1V 0A6, Canada. marie-claude.vohl@fsaa.ulaval.ca.
      sug:
        subj:
          Diet Methods
          Food Habits
          Genetics Physiology
          Adult
          Blood Pressure
          Cereals
          Chronic Disease Prevention and Control
          Cross Sectional Studies
          Female
          Fruit
          Genes
          Human
          Leukocytes, Mononuclear Metabolism
          Male
          Meat
          Middle Age
          Nutrigenomics Methods
          Nutritional Assessment
          Quebec
          Questionnaires
          RNA Metabolism
          Vegetables
          Young Adult
          Funding Source
          Adult: 19-44 years
          Middle Aged: 45-64 years
          Female
          Male
      ab: Background: Diet regulates gene expression profiles by several mechanisms. The objective of this study was to examine gene expression in relation with dietary patterns.Methods: Two hundred and fifty four participants from the greater Quebec City metropolitan area were recruited. Two hundred and ten participants completed the study protocol. Dietary patterns were derived from a food frequency questionnaire (FFQ) by factor analysis. For 30 participants (in fasting state), RNA was extracted from peripheral blood mononuclear cells (PBMCs) and expression levels of 47,231 mRNA transcripts were assessed using the Illumina Human-6 v3 Expression BeadChips®. Microarray data was pre-processed with Flexarray software and analysed with Ingenuity Pathway Analysis (IPA).Results: Two dietary patterns were identified. The Prudent dietary pattern was characterised by high intakes of vegetables, fruits, whole grain products and low intakes of refined grain products and the Western dietary pattern, by high intakes of refined grain products, desserts, sweets and processed meats. When individuals with high scores for the Prudent dietary pattern where compared to individuals with low scores, 2,083 transcripts were differentially expressed in men, 1,136 transcripts in women and 59 transcripts were overlapping in men and women. For the Western dietary pattern, 1,021 transcripts were differentially expressed in men with high versus low scores, 1,163 transcripts in women and 23 transcripts were overlapping in men and women. IPA reveals that genes differentially expressed for both patterns were present in networks related to the immune and/or inflammatory response, cancer and cardiovascular diseases.Conclusion: Gene expression profiles were different according to dietary patterns, which probably modulate the risk of chronic diseases.Trial Registration: NCT: NCT01343342.
      pubtype: Academic Journal
      doctype:
        research
        Journal Article
      ougenre: Article
    language: English
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