The Mannoprotein Cig1 supports iron acquisition from heme and virulence in the pathogenic fungus Cryptococcus neoformans.
Iron acquisition is critical for virulence of the human pathogenic fungus Cryptococcus neoformans. The cryptococcal transcript for the extracellular mannoprotein Cig1 is highly regulated by iron and abundant in iron-starved cells, suggesting a role in iron acquisition. Indeed, loss of Cig1 resulted...
| Publicado en: | Journal of Infectious Diseases Vol. 207; no. 8; pp. 1339 - 1348 |
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| Autores principales: | , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Oxford University Press / USA
Apr2013
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104256575&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104256575 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00221899 1YU jtl: Journal of Infectious Diseases issn: 00221899 maglogo: N pubinfo: dt: Apr2013 vid: 207 iid: 8 pid: 622 pub: Oxford University Press / USA artinfo: ui: 104256575 NLM23322859 2012056285 10.1093/infdis/jit029 NLM23322859 PMC3603535 104256575 ppf: 1339 ppct: 9 formats: tig: atl: The Mannoprotein Cig1 supports iron acquisition from heme and virulence in the pathogenic fungus Cryptococcus neoformans. aug: au: Cadieux, Brigitte Lian, Tianshun Hu, Guanggan Wang, Joyce Biondo, Carmelo Teti, Giuseppe Liu, Victor Murphy, Michael E P Creagh, A Louise Kronstad, James W affil: Michael Smith Laboratories and Department of Microbiology and Immunology, The University of British Columbia, Vancouver, BC, Canada. sug: subj: Cryptococcosis Pathology Cryptococcus Iron Metabolism Metalloporphyrins Metabolism Proteins Metabolism Animal Studies Colony Count, Microbial Cryptococcosis Microbiology Female Genes Hydrogen-Ion Concentration Mice Proteins Recombinant Proteins Recombinant Proteins Metabolism RNA Spectrophotometry Methods Titrimetry Toxins Toxins Metabolism Female ab: Iron acquisition is critical for virulence of the human pathogenic fungus Cryptococcus neoformans. The cryptococcal transcript for the extracellular mannoprotein Cig1 is highly regulated by iron and abundant in iron-starved cells, suggesting a role in iron acquisition. Indeed, loss of Cig1 resulted in delayed growth on heme at physiological pH. Expression of CIG1 is regulated by the pH-responsive transcription factor Rim101, and loss of Rim101 also impaired growth on heme. A cig1Δ mutant was less susceptible than the wild-type strain to noniron metalloporphyrins, further indicating a role for Cig1 in heme uptake. Recombinant Cig1 exhibited the absorbance spectrum of a heme-binding protein upon heme titration, and Cig1 may therefore function as a hemophore at the cell surface. Cig1 contributed to virulence in a mouse model of cryptococcosis but only in a mutant that also lacked the high-affinity iron uptake system. Overall, Cig1-mediated heme uptake is a potential therapeutic target in C. neoformans. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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