High resolution 4-dimension imaging of metanephric embryonic kidney morphogenesis.
High-resolution three-dimensional imaging of fixed embryonic kidney tissues has advanced considerably in the past decade. Here we developed a new process for imaging whole metanephric organ culture at cell resolution in three dimensions over time. This technique combines the use of the newly availab...
| Published in: | Kidney International Vol. 83; no. 4; pp. 757 - 762 |
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| Main Authors: | , , , |
| Format: | Journal Article |
| Published: |
Elsevier B.V.
Apr2013
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104261059&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104261059 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00852538 4CU jtl: Kidney International issn: 00852538 maglogo: N pubinfo: dt: Apr2013 vid: 83 iid: 4 pid: 82545 pub: Elsevier B.V. place: Philadelphia, Pennsylvania artinfo: ui: 104261059 NLM23325081 2012064231 10.1038/ki.2012.464 NLM23325081 PMC3658135 104261059 ppf: 757 ppct: 5 formats: tig: atl: High resolution 4-dimension imaging of metanephric embryonic kidney morphogenesis. aug: au: Clendenon, Sherry G Ward, Heather H Dunn, Kenneth W Bacallao, Robert affil: 1] Department of Physics, Biocomplexity Institute, Indiana University, Bloomington, Indiana, USA [2] Division of Nephrology, Department of Medicine, Indiana University School of Medicine, Indianapolis, Indiana, USA. sug: subj: Diagnostic Imaging Kidney Embryology Microscopy Equipment and Supplies Animals Boron Compounds Metabolism Lipids Metabolism Equipment Design Fluorescent Dyes Metabolism Gestational Age Image Processing, Computer Assisted Kidney Metabolism Mice Morphogenesis Tissue Culture Techniques Time Factors ab: High-resolution three-dimensional imaging of fixed embryonic kidney tissues has advanced considerably in the past decade. Here we developed a new process for imaging whole metanephric organ culture at cell resolution in three dimensions over time. This technique combines the use of the newly available generation of infrared-optimized long working distance, high numerical aperture objectives and multiphoton fluorescence microscopy with a new system for vital staining of metanephric organ cultures with bodipy ceramide. This allows all cells in the organ culture to be visualized over time, enabling detailed observation of tissue morphogenesis. Thus, our method offers a powerful new approach for visualizing and understanding early events in renal development and for extending observations made in genetically manipulated models. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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