Insulin resistance impairs cutaneous wound healing in mice.
Obesity is associated with significant changes in skin combined with metabolic alterations such as insulin resistance. Our aim was to investigate the effects of insulin resistance induced by a high-fat diet on cutaneous wound healing. Male C57BL/6 mice were fed standard chow ( SC group) or high-fat...
| Publicado en: | Wound Repair & Regeneration Vol. 21; no. 3; pp. 464 - 473 |
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| Autores principales: | , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
May/Jun2013
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104286119&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104286119 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10671927 DPV jtl: Wound Repair & Regeneration issn: 10671927 maglogo: Y pubinfo: dt: May/Jun2013 vid: 21 iid: 3 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 104286119 87447401 10.1111/wrr.12042 NLM23627416 104286119 ppf: 464 ppct: 9 formats: fmt: @attributes: type: P tig: atl: Insulin resistance impairs cutaneous wound healing in mice. aug: au: Otranto, Marcela Nascimento, Adriana Paulino do Monte-Alto-Costa, Andréa affil: Department of Histology and Embryology, State University of Rio de Janeiro sug: subj: Insulin Resistance Wound Healing Animal Studies Funding Source Mice Models, Biological Obesity Complications Immunohistochemistry Glucose Tolerance Test Immunoblotting Descriptive Statistics One-Way Analysis of Variance Post Hoc Analysis Mann-Whitney U Test Data Analysis Software ab: Obesity is associated with significant changes in skin combined with metabolic alterations such as insulin resistance. Our aim was to investigate the effects of insulin resistance induced by a high-fat diet on cutaneous wound healing. Male C57BL/6 mice were fed standard chow ( SC group) or high-fat chow ( HFC group) for 30 weeks. On day 0 (28th week), an excisional wound was performed. After 14 days (30th week), the mice were euthanized. Starting from the 8th week, the HFC group had a higher body weight. The HFC group became intolerant to glucose, resistant to insulin, and presented increased plasma cholesterol and triglyceride levels. The wound area was greater in the HFC group. The inflammatory infiltrate and the amount of 'fibroblast-like' cells increased in superficial regions of the lesions in the HFC group. The collagen fibers were more organized and denser in the SC group. Hydroxyproline levels were lower in the HFC group. The nitric oxide synthase-2-positive cells were higher in the HFC group. Lipid peroxidation and protein carbonyl levels were higher in the HFC group. The expression levels of alpha-smooth muscle actin and transforming growth factor-β were higher in the HFC group. These findings support the hypothesis that insulin resistance leads to delayed cutaneous wound healing. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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