MLST genotypes and antibiotic resistance of Campylobacter spp. isolated from poultry in Grenada.
This study determined whether multilocus sequence types (MLST) of Campylobacter from poultry in 2 farms in Grenada, West Indies, differed by farm, antimicrobial resistance and farm antibiotic use. Farm A used fluoroquinolones in the water and Farm B used tetracyclines. The E-test was used to determi...
| Publicado en: | BioMed Research International Vol. 2013; pp. 794643 - 794644 |
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| Autores principales: | , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Wiley-Blackwell
2013
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104287229&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104287229 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 2013 vid: 2013 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 104287229 104287229 2012116822 NLM23555097 PMC3595693 104287229 ppf: 794643 ppct: 1 formats: fmt: @attributes: type: P tig: atl: MLST genotypes and antibiotic resistance of Campylobacter spp. isolated from poultry in Grenada. aug: au: Stone, Diana Davis, Margaret Baker, Katherine Besser, Tom Roopnarine, Rohini Sharma, Ravindra affil: School of Veterinary Medicine, St. George's University, P.O. Box 7, Grenada, West Indies. dstone@sgu.edu sug: subj: Campylobacter Drug Resistance, Microbial Poultry Microbiology Animal Studies Antiinfective Agents, Quinolone Administration and Dosage Poultry Sequence Analysis Tetracyclines Administration and Dosage ab: This study determined whether multilocus sequence types (MLST) of Campylobacter from poultry in 2 farms in Grenada, West Indies, differed by farm, antimicrobial resistance and farm antibiotic use. Farm A used fluoroquinolones in the water and Farm B used tetracyclines. The E-test was used to determine resistance of isolates to seven antibiotics. PCR of the IpxA gene confirmed species and MLST was used to characterize 38 isolates. All isolates were either C. jejuni or C. coli. Farm antibiotic use directly correlated with antimicrobial resistance of Campylobacter isolates. Almost 80% of the isolates from Farm A were fluoroquinolone resistant and 17.9% of the isolates from Farm B were fluoroquinolone resistant. All Campylobacter isolates from Farm A were tetracycline sensitive, whereas 35.7% of isolates from Farm B were tetracycline resistant. Six previously recognized sequence types (STs) and 2 novel STs were identified. Previously recognized STs were those overwhelmingly reported from poultry and humans globally. Isolates with the same ST did not always have the same antibiotic resistance profile. There was little ST overlap between the farms suggesting that within-farm transmission of Campylobacter genotypes may dominate. MLST typing was useful for tracking Campylobacter spp. among poultry units and can help elucidate Campylobacter host-species population structure and its relevance to human health. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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