Titanium surface coating with a laminin-derived functional Peptide promotes bone cell adhesion.
Laminin-derived peptide coatings can enhance epithelial cell adhesion to implants, and the positive effect of these peptides on bone cell adhesion has been anticipated. The purpose of this study was to evaluate the improvement in bone cell attachment to and activity on titanium (Ti) scaffolds coated...
| Publicado en: | BioMed Research International Vol. 2013; pp. 638348 - 638349 |
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| Autores principales: | , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Wiley-Blackwell
2013
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104287275&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104287275 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 2013 vid: 2013 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 104287275 104287275 2012116868 NLM23586052 PMC3622367 104287275 ppf: 638348 ppct: 1 formats: fmt: @attributes: type: P tig: atl: Titanium surface coating with a laminin-derived functional Peptide promotes bone cell adhesion. aug: au: Min, Seung-Ki Kang, Hyun Ki Jang, Da Hyun Jung, Sung Youn Kim, O Bok Min, Byung-Moo Yeo, In-Sung affil: Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University, Seoul 110-749, Republic of Korea. sug: subj: Cell Physiology Drug Effects Dental Implants Osteogenesis Cell Differentiation Cell Line, Tumor Biocompatible Materials Administration and Dosage Biocompatible Materials Human Membrane Proteins Osteosarcoma Metabolism Peptides Administration and Dosage Peptides Surface Properties Titanium Administration and Dosage Titanium ab: Laminin-derived peptide coatings can enhance epithelial cell adhesion to implants, and the positive effect of these peptides on bone cell adhesion has been anticipated. The purpose of this study was to evaluate the improvement in bone cell attachment to and activity on titanium (Ti) scaffolds coated with a laminin-derived functional peptide, Ln2-P3 (the DLTIDDSYWYRI motif). Four Ti disc surfaces were prepared, and a human osteosarcoma (HOS) cell attachment test was performed to select two candidate surfaces for peptide coating. These two candidates were then coated with Ln2-P3 peptide, a scrambled peptide, or left uncoated to measure cell attachment to each surface, following which one surface was chosen to assess alkaline phosphatase (ALP) activity and osteogenic marker gene expression with quantitative real-time PCR. On the commercially pure Ti surface, the Ln2-P3 coating significantly increased cellular ALP activity and the expression levels of ALP and bone sialoprotein mRNA as compared with the scrambled peptide-coated and uncoated surfaces. In conclusion, although further in vivo studies are needed, the findings of this in vitro study indicate that the Ln2-P3-coated implant surface promotes bone cell adhesion, which has clinical implications for reducing the overall treatment time of dental implant therapy. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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