Mass spectrometry-based proteomics in molecular diagnostics: discovery of cancer biomarkers using tissue culture.
Accurate diagnosis and proper monitoring of cancer patients remain a key obstacle for successful cancer treatment and prevention. Therein comes the need for biomarker discovery, which is crucial to the current oncological and other clinical practices having the potential to impact the diagnosis and...
| Publicado en: | BioMed Research International Vol. 2013; pp. 783131 - 783132 |
|---|---|
| Autores principales: | , , , , |
| Formato: | Journal Article |
| Publicado: |
Wiley-Blackwell
2013
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104287282&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104287282 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 2013 vid: 2013 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 104287282 2012116875 NLM23586059 PMC3613068 104287282 ppf: 783131 ppct: 1 formats: fmt: @attributes: type: P tig: atl: Mass spectrometry-based proteomics in molecular diagnostics: discovery of cancer biomarkers using tissue culture. aug: au: Paul, Debasish Kumar, Avinash Gajbhiye, Akshada Santra, Manas K Srikanth, Rapole affil: National Centre for Cell Science, University of Pune Campus, Ganeshkhind, Pune, Maharashtra 411007, India. sug: subj: Neoplasms Diagnosis Neoplasms Pathology, Molecular Proteomics Methods Cell Line, Tumor Electrophoresis Genes Mass Spectrometry Neoplasms Pathology Prognosis Tissue Culture Techniques Tumor Markers, Biological ab: Accurate diagnosis and proper monitoring of cancer patients remain a key obstacle for successful cancer treatment and prevention. Therein comes the need for biomarker discovery, which is crucial to the current oncological and other clinical practices having the potential to impact the diagnosis and prognosis. In fact, most of the biomarkers have been discovered utilizing the proteomics-based approaches. Although high-throughput mass spectrometry-based proteomic approaches like SILAC, 2D-DIGE, and iTRAQ are filling up the pitfalls of the conventional techniques, still serum proteomics importunately poses hurdle in overcoming a wide range of protein concentrations, and also the availability of patient tissue samples is a limitation for the biomarker discovery. Thus, researchers have looked for alternatives, and profiling of candidate biomarkers through tissue culture of tumor cell lines comes up as a promising option. It is a rich source of tumor cell-derived proteins, thereby, representing a wide array of potential biomarkers. Interestingly, most of the clinical biomarkers in use today (CA 125, CA 15.3, CA 19.9, and PSA) were discovered through tissue culture-based system and tissue extracts. This paper tries to emphasize the tissue culture-based discovery of candidate biomarkers through various mass spectrometry-based proteomic approaches. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|