Identification of a novel bone morphogenetic protein (BMP)-inducible transcript, BMP-inducible transcript-1, by utilizing the conserved BMP-responsive elements in the Id genes.
Bone morphogenetic proteins (BMPs) inhibit myogenesis and induce osteoblastic differentiation in myoblasts. They also induce the transcription of several common genes, such as Id1, Id2 and Id3, in various cell types. We have reported that a GC-rich element in the Id1 gene functions as a BMP-responsi...
| Publicado en: | Journal of Bone & Mineral Metabolism Vol. 31; no. 1; pp. 34 - 44 |
|---|---|
| Autores principales: | , , , , , , , , , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Springer Nature
Jan2013
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104294807&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104294807 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09148779 NR4 jtl: Journal of Bone & Mineral Metabolism issn: 09148779 maglogo: N pubinfo: dt: Jan2013 vid: 31 iid: 1 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 104294807 NLM22976053 2011900167 10.1007/s00774-012-0381-1 NLM22976053 104294807 ppf: 34 ppct: 10 formats: tig: atl: Identification of a novel bone morphogenetic protein (BMP)-inducible transcript, BMP-inducible transcript-1, by utilizing the conserved BMP-responsive elements in the Id genes. aug: au: Shin M Ohte S Fukuda T Sasanuma H Yoneyama K Kokabu S Miyamoto A Tsukamoto S Hohjoh H Jimi E Katagiri T Shin, Masashi Ohte, Satoshi Fukuda, Toru Sasanuma, Hiroki Yoneyama, Katsumi Kokabu, Shoichiro Miyamoto, Arei Tsukamoto, Sho Hohjoh, Hirohiko affil: Division of Pathophysiology, Research Center for Genomic Medicine, Saitama Medical University, 1397-1 Yamane, Hidaka-shi, Saitama, 350-1241, Japan sug: subj: Bone Morphogenetic Proteins Metabolism DNA Physiology Muscle Proteins Metabolism Proteins RNA Metabolism Signal Transduction Physiology Stem Cells Metabolism Animal Studies Bone Morphogenetic Proteins Cells Chromosomes Chromosomes Metabolism Immunity Mice Muscle Proteins RNA ab: Bone morphogenetic proteins (BMPs) inhibit myogenesis and induce osteoblastic differentiation in myoblasts. They also induce the transcription of several common genes, such as Id1, Id2 and Id3, in various cell types. We have reported that a GC-rich element in the Id1 gene functions as a BMP-responsive element (BRE) that is regulated by Smads. In this study, we analyzed and identified BREs in the 5'-flanking regions of the mouse Id2 and Id3 genes. The core GGCGCC sequence was conserved among the BREs in the Id1, Id2 and Id3 genes and was essential for the response to BMP signaling via Smads. We found a novel BRE on mouse chromosome 13 at position 47,723,740-47,723,768 by searching for conserved sequences containing the Id1 BRE. This potential BRE was found in the 5'-flanking region of a novel gene that produces a non-coding transcript, termed BMP-inducible transcript-1 (BIT-1), and this element regulated the expression of this gene in response to BMP signaling. We found that BIT-1 is expressed in BMP target tissues such as the testis, brain, kidney and cartilage. These findings suggest that the transcriptional induction of the Ids, BIT-1 and additional novel genes containing the conserved BRE sequence may play an important role in the regulation of the differentiation and/or function of target cells in response to BMPs. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|