Expression of Neural Markers by Undifferentiated Rat Mesenchymal Stem Cells.
The spontaneous expression of neural markers by mesenchymal stem cells (MSCs) has been considered to be a demonstration of MSCs' predisposition to differentiate towards neural lineages. In view of their application in cell therapy for neurodegenerative diseases, it is very important to deepen the kn...
| Published in: | Journal of Biomedicine & Biotechnology Vol. 2012; pp. 1 - 14 |
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| Main Authors: | , , , , , |
| Format: | pictorial research tables/charts Journal Article |
| Published: |
Wiley-Blackwell
2012
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104297837&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104297837 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 11107243 137K jtl: Journal of Biomedicine & Biotechnology issn: 11107243 maglogo: N pubinfo: dt: 2012 vid: 2012 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 104297837 104297837 2011906759 NLM23093867 PMC3474592 104297837 ppf: 1 ppct: 13 formats: fmt: @attributes: type: P tig: atl: Expression of Neural Markers by Undifferentiated Rat Mesenchymal Stem Cells. aug: au: Foudah, Dana Redondo, Juliana Caldara, Cristina Carini, Fabrizio Tredici, Giovanni Miloso, Mariarosaria affil: Dipartimento di Neuroscienze e Tecnologie Biomediche, Facoltà di Medicina e Chirurgia, Università degli Studi di Milano-Bicocca, Via Cadore 48, 20052 Monza, Italy sug: subj: Stem Cells Physiology Biological Markers Cell Differentiation Stem Cells Metabolism Animal Studies Rats Nerve Tissue Proteins Metabolism Neurons Physiology Neurons Metabolism Immunoblotting One-Way Analysis of Variance Descriptive Statistics Post Hoc Analysis ab: The spontaneous expression of neural markers by mesenchymal stem cells (MSCs) has been considered to be a demonstration of MSCs' predisposition to differentiate towards neural lineages. In view of their application in cell therapy for neurodegenerative diseases, it is very important to deepen the knowledge about this distinctive biological property of MSCs. In this study, we evaluated the expression of neuronal and glial markers in undifferentiated rat MSCs (rMSCs) at different culture passages (from early to late). rMSCs spontaneously expressed neural markers depending on culture passage, and they were coexpressed or not with the neural progenitor marker nestin. In contrast, the number of rMSCs expressing mesengenic differentiation markers was very low or even completely absent. Moreover, rMSCs at late culture passages were not senescent cells and maintained the MSC immunophenotype. However, their differentiation capabilities were altered. In conclusion, our results support the concept of MSCs as multidifferentiated cells and suggest the existence of immature and mature neurally fated rMSC subpopulations. A possible correlation between specific MSC subpopulations and specific neural lineages could optimize the use of MSCs in cell transplantation therapy for the treatment of neurological diseases. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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