Investigation of Antimicrobial Activity and Statistical Optimization of Bacillus subtilis SPB1 Biosurfactant Production in Solid-State Fermentation.
During the last years, several applications of biosurfactants with medical purposes have been reported. Biosurfactants are considered relevant molecules for applications in combating many diseases. However, their use is currently extremely limited due to their high cost in relation to that of chemic...
| Publicado en: | Journal of Biomedicine & Biotechnology Vol. 2012; pp. 1 - 13 |
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| Autores principales: | , , , , , , , |
| Formato: | equations & formulas research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
2012
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104298118&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104298118 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 11107243 137K jtl: Journal of Biomedicine & Biotechnology issn: 11107243 maglogo: N pubinfo: dt: 2012 vid: 2012 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 104298118 104298118 2011907038 NLM22536017 PMC3321739 104298118 ppf: 1 ppct: 12 formats: fmt: @attributes: type: P tig: atl: Investigation of Antimicrobial Activity and Statistical Optimization of Bacillus subtilis SPB1 Biosurfactant Production in Solid-State Fermentation. aug: au: Ghribi, Dhouha Abdelkefi-Mesrati, Lobna Mnif, Ines Kammoun, Radhouan Ayadi, Imen Saadaoui, Imen Maktouf, Sameh Chaabouni-Ellouze, Semia affil: Unité Enzyme et Bioconversion, Ecole Nationale d'Ingénieurs de Sfax, BP W, 3038 Sfax, Tunisia; Institut Supérieur de Biotechnologie de Sfax, BP 261, 3000 Sfax, Tunisia sug: subj: Antiinfective Agents Pharmacodynamics Bacillus Drug Effects Surface-Active Agents Pharmacodynamics Models, Statistical Fermentation Drug Resistance, Microbial Drug Effects Polymerase Chain Reaction Cell Culture Techniques Descriptive Statistics Analysis of Variance Data Analysis Software Regression Fisher's Exact Test Post Hoc Analysis Funding Source ab: During the last years, several applications of biosurfactants with medical purposes have been reported. Biosurfactants are considered relevant molecules for applications in combating many diseases. However, their use is currently extremely limited due to their high cost in relation to that of chemical surfactants. Use of inexpensive substrates can drastically decrease its production cost. Here, twelve solid substrates were screened for the production of Bacillus subtilis SPB1 biosurfactant and the maximum yield was found with millet. A Plackett-Burman design was then used to evaluate the effects of five variables (temperature, moisture, initial pH, inoculum age, and inoculum size). Statistical analyses showed that temperature, inoculum age, and moisture content had significantly positive effect on SPB1 biosurfactant production. Their values were further optimized using a central composite design and a response surface methodology. The optimal conditions of temperature, inoculum age, and moisture content obtained under the conditions of study were 37?C, 14 h, and 88%, respectively. The evaluation of the antimicrobial activity of this compound was carried out against 11 bacteria and 8 fungi. The results demonstrated that this biosurfactant exhibited an important antimicrobial activity against microorganisms with multidrug-resistant profiles. Its activity was very effective against Staphylococcus aureus, Staphylococcus xylosus, Enterococcus faecalis, Klebsiella pneumonia, and so forth. pubtype: Academic Journal doctype: equations & formulas research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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