AngiotensinII Preconditioning Promotes Angiogenesis In Vitro via ERKs Phosphorylation.
AngiotensinII (AngII) is involved in not only the formation of cardiac hypertrophy but also the development of cardiac remodeling both of which are associated with myocardial angiogenesis. This study was therefore performed to clarify the effects of AngII on the formation of vasculatures by cultured...
| Publicado en: | Journal of Biomedicine & Biotechnology Vol. 2012; pp. 1 - 7 |
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| Autores principales: | , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
2012
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104298157&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104298157 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 11107243 137K jtl: Journal of Biomedicine & Biotechnology issn: 11107243 maglogo: N pubinfo: dt: 2012 vid: 2012 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 104298157 104298157 2011907079 NLM22500105 PMC3303689 104298157 ppf: 1 ppct: 6 formats: fmt: @attributes: type: P tig: atl: AngiotensinII Preconditioning Promotes Angiogenesis In Vitro via ERKs Phosphorylation. aug: au: Aili Guan Yunzeng Zou Hui Gong Yuhong Niu Yong Ye Jianguo Jia Bingyu Li Guoping Zhang Sanli Qian Junbo Ge affil: Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, 180 Feng Lin Road, Shanghai 200032, China sug: subj: Growth Substances Pharmacodynamics Angiotensin II Pharmacodynamics Transferases Antagonists and Inhibitors Transferases Metabolism Neovascularization, Physiologic Drug Effects In Vitro Studies Epithelial Cells Drug Effects Epithelial Cells Metabolism Rats Animal Studies Myocardium Anatomy and Histology Descriptive Statistics Analysis of Variance Data Analysis Software Funding Source ab: AngiotensinII (AngII) is involved in not only the formation of cardiac hypertrophy but also the development of cardiac remodeling both of which are associated with myocardial angiogenesis. This study was therefore performed to clarify the effects of AngII on the formation of vasculatures by cultured cardiac microvascular endothelial cells (CMVECs) after a long-period stimulation with or without the AngII preconditioning. Incubation with AngII for 18 hrs significantly impaired the formation of capillary-like tubes comparing to that without AngII. CMVECs with AngII pretreatment for 5 and 10min formed more capillary-like tubes than those without AngII pretreatment, suggesting that preconditioning with AngII at a lower dose for a short period could prevent the further damage of CMVECs by a higher concentration of AngII. Moreover, AngII (10-7 M) stimulation for 5 and 10 min significantly induced the increase in extracellular signal-regulated protein kinases (ERKs) phosphorylation, and an ERKs inhibitor, PD98059, abrogated the increase in the formation of capillary-like tubes induced by the AngII-pretreatment. In conclusion, preconditioning with a lower concentration of AngII for a short period prevents the subsequent impairment of CMVECs by a higher dose of AngII, at least in part, through the increase in ERKs phosphorylation. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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