Micromechanical Thermal Assays of Ca2+-Regulated Thin-Filament Function and Modulation by Hypertrophic Cardiomyopathy Mutants of Human Cardiac Troponin.
Microfabricated thermoelectric controllers can be employed to investigate mechanisms underlying myosin-driven sliding of Ca2+- regulated actin and disease-associated mutations in myofilament proteins. Specifically, we examined actin filament sliding—with or without human cardiac troponin (Tn) and α-...
| Published in: | Journal of Biomedicine & Biotechnology Vol. 2012; pp. 1 - 14 |
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| Main Authors: | , , , , , , |
| Format: | equations & formulas pictorial research tables/charts Journal Article |
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Wiley-Blackwell
2012
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104298185&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104298185 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 11107243 137K jtl: Journal of Biomedicine & Biotechnology issn: 11107243 maglogo: N pubinfo: dt: 2012 vid: 2012 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 104298185 104298185 2011907106 104298185 ppf: 1 ppct: 13 formats: fmt: @attributes: type: P tig: atl: Micromechanical Thermal Assays of Ca2+-Regulated Thin-Filament Function and Modulation by Hypertrophic Cardiomyopathy Mutants of Human Cardiac Troponin. aug: au: Brunet, Nicolas M. Mihajlović, Goran Aledealat, Khaled Fang Wang Peng Xiong von Molnár, Stephan Chase, P. Bryant affil: Department of Biological Science, Florida State University, P.O. Box 3064295, Tallahassee, FL 32306, USA; Institute of Molecular Biophysics, Florida State University, Tallahassee, FL 32306, USA; Donders Institute for Brain, Cognition and Behavior, Centre for Cognitive Neuroimaging, Radboud University Nijmegen, 6500 HB Nijmegen, The Netherlands sug: subj: Calcium Metabolism Chemistry, Analytical Cardiomyopathy, Hypertrophic Familial and Genetic Cardiomyopathy, Hypertrophic Metabolism Troponin Metabolism Temperature Muscle Proteins Metabolism Adenosine Triphosphatase Metabolism Microfilament Proteins Metabolism Models, Biological Rabbits Animal Studies Human In Vitro Studies Descriptive Statistics Data Analysis Software Linear Regression Models, Statistical Funding Source ab: Microfabricated thermoelectric controllers can be employed to investigate mechanisms underlying myosin-driven sliding of Ca2+- regulated actin and disease-associated mutations in myofilament proteins. Specifically, we examined actin filament sliding—with or without human cardiac troponin (Tn) and α-tropomyosin (Tm)—propelled by rabbit skeletal heavy meromyosin, when temperature was varied continuously over a wide range (∼20-63°C). At the upper end of this temperature range, reversible dysregulation of thin filaments occurred at pCa 9 and 5; actomyosin function was unaffected. Tn-Tm enhanced sliding speed at pCa 5 and increased a transition temperature (Tt) between a high activation energy (Ea) but low temperature regime and a low Ea but high temperature regime. This was modulated by factors that alter cross-bridge number and kinetics. Three familial hypertrophic cardiomyopathy (FHC) mutations, cTnI R145G, cTnI K206Q, and cTnT R278C, cause dysregulation at temperatures ∼5-8°C lower; the latter two increased speed at pCa 5 at all temperatures. pubtype: Academic Journal doctype: equations & formulas pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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