Detection of Target ssDNA Using a Microfabricated Hall Magnetometer with Correlated Optical Readout.
Sensing biological agents at the genomic level, while enhancing the response time for biodetection over commonly used, optics-based techniques such as nucleic acid microarrays or enzyme-linked immunosorbent assays (ELISAs), is an important criterion for new biosensors. Here, we describe the successf...
| Publicado en: | Journal of Biomedicine & Biotechnology Vol. 2012; pp. 1 - 11 |
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| Autores principales: | , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
2012
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104298190&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104298190 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 11107243 137K jtl: Journal of Biomedicine & Biotechnology issn: 11107243 maglogo: N pubinfo: dt: 2012 vid: 2012 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 104298190 104298190 2011907111 NLM22496610 PMC3303874 104298190 ppf: 1 ppct: 10 formats: fmt: @attributes: type: P tig: atl: Detection of Target ssDNA Using a Microfabricated Hall Magnetometer with Correlated Optical Readout. aug: au: Hira, Steven M. Aledealat, Khaled Kan-Sheng Chen Field, Mark Sullivan, Gerard J. Chase, P. Bryant Peng Xiong von Molnár, Stephan Strouse, Geoffrey F. affil: Department of Chemistry and Biochemistry, Florida State University, Tallahassee, FL 32306-4390, USA sug: subj: DNA Analysis Magnetics Methods Microscopy Methods Biosensing Techniques Equipment and Supplies Equipment Design Sensitivity and Specificity Microscopy Equipment and Supplies Oligonucleotide Array Sequence Analysis Funding Source ab: Sensing biological agents at the genomic level, while enhancing the response time for biodetection over commonly used, optics-based techniques such as nucleic acid microarrays or enzyme-linked immunosorbent assays (ELISAs), is an important criterion for new biosensors. Here, we describe the successful detection of a 35-base, single-strand nucleic acid target by Hall-based magnetic transduction as a mimic for pathogenic DNA target detection. The detection platform has low background, large signal amplification following target binding and can discriminate a single, 350nm superparamagnetic bead labeled with DNA. Detection of the target sequence was demonstrated at 364pM (<2 target DNA strands per bead) target DNA in the presence of 36 μM nontarget (noncomplementary) DNA (<10 ppm target DNA) using optical microscopy detection on a GaAs Hall mimic. The use of Hall magnetometers as magnetic transduction biosensors holds promise for multiplexing applications that can greatly improve point-of-care (POC) diagnostics and subsequent medical care. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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