Thin-Layer Hydroxyapatite Deposition on a Nanofiber Surface Stimulates Mesenchymal Stem Cell Proliferation and Their Differentiation into Osteoblasts.
Pulsed laser deposition was proved as a suitable method for hydroxyapatite (HA) coating of coaxial poly-e-caprolactone/polyvinylalcohol (PCL/PVA) nanofibers. The fibrous morphology of PCL/PVA nanofibers was preserved, if the nanofiber scaffold was coated with thin layers of HA (200nm and 400 nm). In...
| Publicado en: | Journal of Biomedicine & Biotechnology Vol. 2012; pp. 1 - 11 |
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| Autores principales: | , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
2012
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104298210&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104298210 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 11107243 137K jtl: Journal of Biomedicine & Biotechnology issn: 11107243 maglogo: N pubinfo: dt: 2012 vid: 2012 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 104298210 104298210 2011907132 NLM22319242 PMC3272836 104298210 ppf: 1 ppct: 10 formats: fmt: @attributes: type: P tig: atl: Thin-Layer Hydroxyapatite Deposition on a Nanofiber Surface Stimulates Mesenchymal Stem Cell Proliferation and Their Differentiation into Osteoblasts. aug: au: Prosecká, Eva Buzgo, Matej Rampichová, Michala Kocourek, Tomáš Kochová, Petra Vysloužilová, Lucie Tvrdík, Daniel Jelínek, Miroslav Lukáš, David Amler, Evžen affil: Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Science of the Czech Republic, Videnska 1083, 142 40 Prague, Czech Republic; Department of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, V Uvalu 84, 150 06 Prague 5, Czech Republic sug: subj: Cell Differentiation Drug Effects Stem Cells Connective Tissue Cells Cell Physiology Drug Effects Minerals Pharmacodynamics Polyvinyls Animal Studies Swine Polymerase Chain Reaction Descriptive Statistics One-Way Analysis of Variance T-Tests Post Hoc Analysis Funding Source ab: Pulsed laser deposition was proved as a suitable method for hydroxyapatite (HA) coating of coaxial poly-e-caprolactone/polyvinylalcohol (PCL/PVA) nanofibers. The fibrous morphology of PCL/PVA nanofibers was preserved, if the nanofiber scaffold was coated with thin layers of HA (200nm and 400 nm). Increasing thickness of HA, however, resulted in a gradual loss of fibrous character. In addition, biomechanical properties were improved after HA deposition on PCL/PVA nanofibers as the value of Young's moduli of elasticity significantly increased. Clearly, thin-layer hydroxyapatite deposition on a nanofiber surface stimulated mesenchymal stem cell viability and their differentiation into osteoblasts. The optimal depth of HA was 800 nm. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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