Influences of Desmin and Keratin 19 on Passive Biomechanical Properties of Mouse Skeletal Muscle.

In skeletal muscle fibers, forces must be transmitted between the plasma membrane and the intracellular contractile lattice, and within this lattice between adjacent myofibrils. Based on their prevalence, biomechanical properties and localization, desmin and keratin intermediate filaments (IFs) are...

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Publicado en:Journal of Biomedicine & Biotechnology Vol. 2012; pp. 1 - 13
Autores principales: Shah, Sameer B., Love, James M., O'Neill, Andrea, Lovering, Richard M., Bloch, Robert J.
Formato: pictorial research tables/charts Journal Article
Publicado: Wiley-Blackwell 2012
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 2012
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      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        atl: Influences of Desmin and Keratin 19 on Passive Biomechanical Properties of Mouse Skeletal Muscle.
      aug:
        au:
          Shah, Sameer B.
          Love, James M.
          O'Neill, Andrea
          Lovering, Richard M.
          Bloch, Robert J.
        affil: Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA; Departments of Orthopaedic Surgery and Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA
      sug:
        subj:
          Muscle, Skeletal Physiology
          Cytoskeletal Proteins Physiology
          Cell Membrane Physiology
          Muscle Fibers Physiology
          Animal Studies
          Mice
          Regression
          Analysis of Variance
          Post Hoc Analysis
          Two-Tailed Test
          Descriptive Statistics
          Data Analysis Software
          Muscular Diseases Familial and Genetic
          Muscular Diseases Pathology
          Weight-Bearing
          Cytoplasm Physiology
          Funding Source
      ab: In skeletal muscle fibers, forces must be transmitted between the plasma membrane and the intracellular contractile lattice, and within this lattice between adjacent myofibrils. Based on their prevalence, biomechanical properties and localization, desmin and keratin intermediate filaments (IFs) are likely to participate in structural connectivity and force transmission. We examined the passive load-bearing response of single fibers from the extensor digitorum longus (EDL) muscles of young (3 months) and aged (10months) wild-type, desmin-null, K19-null, and desmin/K19 double-null mice. Though fibers are more compliant in allmutant genotypes compared to wild-type, the structural response of each genotype is distinct, suggesting multiple mechanisms by which desmin and keratin influence the biomechanical properties of myofibers. This work provides additional insight into the influences of IFs on structure-function relationships in skeletal muscle. It may also have implications for understanding the progression of desminopathies and other IF-related myopathies.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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