In vivo magnetic resonance imaging of transgenic mice expressing human ferritin.
Objective: This study aims to produce the transgenic mice (TG) engineered for magnetic resonance imaging (MRI) studies based on the ubiquitous expression of ferritin MRI reporter gene in diverse tissues.Procedures: Transgenic mice (TG) expressing myc-tagged human heavy chain ferritin (myc-hFTH) unde...
| Publicado en: | Molecular Imaging & Biology Vol. 15; no. 1; pp. 48 - 58 |
|---|---|
| Autores principales: | , , , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Springer Nature
Feb2013
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104301099&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104301099 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15361632 KJU jtl: Molecular Imaging & Biology issn: 15361632 maglogo: N pubinfo: dt: Feb2013 vid: 15 iid: 1 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 104301099 NLM22733452 2011910768 10.1007/s11307-012-0567-x NLM22733452 104301099 ppf: 48 ppct: 10 formats: fmt: @attributes: type: P tig: atl: In vivo magnetic resonance imaging of transgenic mice expressing human ferritin. aug: au: Kim HS Joo HJ Woo JS Choi YS Choi SH Kim H Moon WK Kim, Hoe Suk Joo, Hyun Jung Woo, Ji Su Choi, Yoon Seok Choi, Seung Hong Kim, Hyeonjin Moon, Woo Kyung affil: Department of Radiology, Seoul National University Hospital, 101 Daehangno, Jongno-gu, Seoul, 110-744, Korea sug: subj: Ferritin Analysis Magnetic Resonance Imaging Methods Molecular Imaging Methods Recombinant Proteins Analysis Analysis of Variance Animal Studies Biochemical Phenomena Brain Metabolism Brain Pathology Epithelial Cells Female Ferritin Genetic Techniques Immunity In Vivo Studies Iron Iron Analysis Iron Metabolism Liver Liver Metabolism Liver Pathology Male Mice Recombinant Proteins Spleen Spleen Metabolism Spleen Pathology Female Male ab: Objective: This study aims to produce the transgenic mice (TG) engineered for magnetic resonance imaging (MRI) studies based on the ubiquitous expression of ferritin MRI reporter gene in diverse tissues.Procedures: Transgenic mice (TG) expressing myc-tagged human heavy chain ferritin (myc-hFTH) under the control of a ubiquitous CAG promoter were produced. The expression of myc-hFTH in diverse tissues of the myc-hFTH TG was assessed by RT-PCR, Western blotting, and immunohistochemistry. The iron accumulation and the deposition of ferritin aggregates in tissues of myc-hFTH TG and WT were analyzed by Prussian blue staining and transmission electron microscopy. The myc-hFTH TG (n = 9) and wild-type mice (WT) (n = 4) were subjected to MRI on 9.4 T MR scanner. An eight-point T(2)* mapping was performed using a multiple gradient echo sequence, and T(2)* value was estimated pixel by pixel by using a routine least-squares fitting algorithm.Results: We generated the myc-hFTH TG expressing myc-hFTH in brain, heart, liver, lung, spleen, pancreas, kidney, and intestine. The myc-hFTH TG showed no apparent pathological symptoms and no histological changes compared to WT. The expression of myc-hFTH in the brain and liver tissues of myc-hFTH TG led to a significant decrease in T(2)* values, as shown by noninvasive MRI, compared to WT (P < 0.05, TG vs. WT).Conclusions: This study demonstrates that the novel myc-hFTH TG, which expresses an MRI reporter in many tissues, would be a valuable animal model of FTH-based molecular imaging in which to study potential therapies for cell and tissue grafting using an MRI technique. These mice could also serve to study disease related with iron metabolism. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|