Release of growth factors and the effect of age, sex, and severity of injury after long bone fracture.
Background and purpose The systemic response after fracture is regulated by a complex mechanism involving numerous growth factors. In this study, we analyzed the kinetics of key growth factors following lower-limb long bone fracture. Materials and methods Human serum was isolated from 15 patients su...
| Publicado en: | Acta Orthopaedica Vol. 84; no. 1; pp. 65 - 71 |
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| Autores principales: | , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Medical Journals Sweden AB
Feb2013
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104314078&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104314078 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 17453674 1BVL jtl: Acta Orthopaedica issn: 17453674 maglogo: N pubinfo: dt: Feb2013 vid: 84 iid: 1 pid: 59195 pub: Medical Journals Sweden AB artinfo: ui: 104314078 85463981 10.3109/17453674.2013.765624 NLM23343371 104314078 ppf: 65 ppct: 6 formats: fmt: @attributes: type: P tig: atl: Release of growth factors and the effect of age, sex, and severity of injury after long bone fracture. aug: au: Pountos, Ippokratis Georgouli, Theodora Henshaw, Karen Bird, Howard Giannoudis, Peter V affil: Academic Department of Trauma and Orthopaedics sug: subj: Tibial Fractures Surgery Growth Substances Blood Femoral Fractures Surgery Human Descriptive Statistics Data Analysis Software Biological Markers Male Female Adult Middle Age Aged Control Group Analysis of Variance Pearson's Correlation Coefficient T-Tests Mann-Whitney U Test Confidence Intervals Adult: 19-44 years Middle Aged: 45-64 years Aged: 65+ years Male Female ab: Background and purpose The systemic response after fracture is regulated by a complex mechanism involving numerous growth factors. In this study, we analyzed the kinetics of key growth factors following lower-limb long bone fracture. Materials and methods Human serum was isolated from 15 patients suffering from lower-limb long bone fracture (tibia/femur) requiring surgical fixation. The levels of platelet-derived growth factor (PDGF-BB), vascular edothelial growth factor (VEGF), insulin growth factor-I (IGF-I), and transforming growth factor β1 (TGF-β1) were assayed by colorimetric ELISA at different time points during the first week after fracture. 10 healthy volunteers made up the control group of the study. Serum levels of the growth factors measured were compared to age, sex, and injury severity score. Results We found that there was a decline in the levels of PDGF-BB, IGF-I and TGF-β1 during the first 3 days after fracture. However, VEGF levels remained unchanged. The levels of all the growth factors studied then increased, with the highest concentrations noted at day 7 after surgery. No correlation was found between circulating levels of growth factors and age, injury severity score (ISS), blood loss, or fluid administration. Interpretation There are systemic mitogenic and osteogenic signals after fracture. Important growth factors are released into the peripheral circulation, but early after surgery it appears that serum levels of key growth factors fall. By 7 days postoperatively, the levels had increased considerably. Our findings should be considered in cases where autologous serum is used for ex vivo expansion of mesenchymal stem cells. There should be further evaluation of the use of these molecules as biomarkers of bone union. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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