Evaluation of Microfluidic Biosensor Development Using Microscopic Analysis of Molecular Beacon Hybridization Kinetics.
Abstract Molecular beacons, oligonucleotide probes that fluoresce upon hybridization to a target nucleic acid, can be used in microfluidic devices to detect and quantify nucleic acids in solution as well as inside bacterial cells. Three essential steps towards the development of such devices as inte...
| Publicado en: | Biomedical Microdevices Vol. 7; no. 1; pp. 7 - 13 |
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| Autores principales: | , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
Mar2005
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104322584&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104322584 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 13872176 ODN jtl: Biomedical Microdevices issn: 13872176 maglogo: N pubinfo: dt: Mar2005 vid: 7 iid: 1 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 104322584 2011941001 104322584 ppf: 7 ppct: 6 formats: fmt: @attributes: type: P tig: atl: Evaluation of Microfluidic Biosensor Development Using Microscopic Analysis of Molecular Beacon Hybridization Kinetics. aug: au: Chuanwu Xi Lutgarde Raskin Stephen A. Boppart affil: Department of Civil and Environmental Engineering University of Illinois at Urbana-Champaign, Beckman Institute for Advanced Science and Technology Urbana IL 61801 USA sug: ab: Abstract Molecular beacons, oligonucleotide probes that fluoresce upon hybridization to a target nucleic acid, can be used in microfluidic devices to detect and quantify nucleic acids in solution as well as inside bacterial cells. Three essential steps towards the development of such devices as integrated microfluidic biosensors using molecular beacons were investigated in the present study. First, experiments using real-time confocal microscopy indicated that diffusion of DNA molecular beacons across a 100-m diameter microfluidic channel took less than one minute after the flow of reagents was stopped. Second, experiments to evaluate hybridization kinetics of DNA molecular beacons with target nucleic acids in solution showed that DNA molecular beacons can be used to characterize hybridization kinetics in real time in microfluidic channels and that hybridization signals approached their maximum in approximately three minutes. Finally, it was demonstrated that peptide nucleic acid molecular beacons can be used to detect bacterial cells in microfluidic devices. These results suggest that the use of microfluidic devices to detect nucleic acids in solution and in bacterial cells is promising and that further development of an integrated microfluidic biosensor for bacterial detection based on this concept is warranted. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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