Determination of electrode to nerve fiber distance and nerve conduction velocity through spectral analysis of the extracellular action potentials recorded from earthworm giant fibers.

Microneurography and the use of selective microelectrodes that can resolve single-unit nerve activity have become a tool to understand the coding within the nervous system and a clinical diagnostic tool to assess peripheral neural pathologies. Central to these techniques is the use of the difference...

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Publicado en:Medical & Biological Engineering & Computing Vol. 50; no. 8; pp. 867 - 876
Autores principales: Qiao S, Odoemene O, Yoshida K, Qiao, Shaoyu, Odoemene, Onyekachi, Yoshida, Ken
Formato: research Journal Article
Publicado: Springer Nature Aug2012
Acceso en línea:Ver este registro en EBSCOhost
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        atl: Determination of electrode to nerve fiber distance and nerve conduction velocity through spectral analysis of the extracellular action potentials recorded from earthworm giant fibers.
      aug:
        au:
          Qiao S
          Odoemene O
          Yoshida K
          Qiao, Shaoyu
          Odoemene, Onyekachi
          Yoshida, Ken
        affil: Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA
      sug:
        subj:
          Algorithms
          Electric Stimulation Equipment and Supplies
          Electrodes
          Models, Biological
          Nerve Fibers Physiology
          Neural Conduction Physiology
          Invertebrates Physiology
          Animals
          Computer Simulation
          Interstitial Fluid Physiology
      ab: Microneurography and the use of selective microelectrodes that can resolve single-unit nerve activity have become a tool to understand the coding within the nervous system and a clinical diagnostic tool to assess peripheral neural pathologies. Central to these techniques is the use of the differences in the shape of the extracellular action potential (AP) waveform to identify and discriminate units from one another. Theoretical modeling of the origins of these shape differences has shown that the position of the nerve fiber relative to the electrode and the conduction velocity of the unit contribute to these differences giving rise to the hypothesis that more information about the fiber and its relationship to the electrode could be extracted given further analysis of the AP waveform. This paper addresses this question by exploring the electrical coupling between the electrode and nerve fiber. Idealized models and the literature indicate that two parameters, the electrode-fiber distance and the unit conduction velocity, contribute to the amplitude of the extracellular AP detected by the electrode, which confounds the quantification of coupling using the spike amplitude alone. To resolve this, we develop a method that enables differential quantification of these two parameters using spectral analysis of the single-unit AP waveform and demonstrate that the two parameters could be effectively decoupled in an in vitro earthworm model. The method could open the way forward toward micro-scale in situ monitoring of the interaction of nerve fiber and neural interface.
      pubtype: Academic Journal
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        research
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    language: English
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