Live Helicobacter pylori in the root canal of endodontic-infected deciduous teeth.
Background: Many polymerase chain reaction (PCR)-based studies have shown that Helicobacter pylori DNA is prevalent in the oral cavity, but reports on the isolation of live bacteria are extremely rare. Thus, it is still unclear whether H. pylori can indeed survive in the oral environment.Methods: He...
| Publicado en: | Journal of Gastroenterology Vol. 47; no. 8; pp. 936 - 941 |
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| Autores principales: | , , , , , , , , , , , |
| Formato: | case study Journal Article |
| Publicado: |
Springer Nature
Aug2012
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104362719&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104362719 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09441174 EJ7 jtl: Journal of Gastroenterology issn: 09441174 maglogo: N pubinfo: dt: Aug2012 vid: 47 iid: 8 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 104362719 NLM22722905 2011660220 10.1007/s00535-012-0618-8 NLM22722905 104362719 ppf: 936 ppct: 5 formats: tig: atl: Live Helicobacter pylori in the root canal of endodontic-infected deciduous teeth. aug: au: Hirsch C Tegtmeyer N Rohde M Rowland M Oyarzabal OA Backert S Hirsch, Christian Tegtmeyer, Nicole Rohde, Manfred Rowland, Marion Oyarzabal, Omar A Backert, Steffen affil: Department of Paediatric Dentistry, University School of Dental Medicine, University of Leipzig, Nuernberger Straße 57, 04103, Leipzig, Germany sug: subj: Dental Caries Microbiology Dental Plaque Microbiology Dental Pulp Cavity Microbiology Helicobacter Infections Microbiology Helicobacter Pylori Child, Preschool Male Microscopy, Electron, Scanning Polymerase Chain Reaction Tooth, Deciduous Microbiology Child, Preschool: 2-5 years Male ab: Background: Many polymerase chain reaction (PCR)-based studies have shown that Helicobacter pylori DNA is prevalent in the oral cavity, but reports on the isolation of live bacteria are extremely rare. Thus, it is still unclear whether H. pylori can indeed survive in the oral environment.Methods: Here we used electron microscopy, selective growth techniques, urease assays, 16S rRNA PCR, and western blotting to investigate the possible presence of live H. pylori in 10 root canal and corresponding plaque samples of endodontic-infected deciduous teeth in three children.Results: Although H. pylori DNA was verifiable by PCR in several plaque and root canal samples, bacterial colonies could only be grown from two root canals, but not from plaque. These colonies were unequivocally identified as H. pylori by microscopic, genetic, and biochemical approaches.Conclusions: Our findings show that root canals of endodontic-infected teeth may be a reservoir for live H. pylori that could serve as a potential source for transmission. pubtype: Academic Journal doctype: case study Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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