Real-imaging cDNA-AFLP transcript profiling of pancreatic cancer patients: Egr-1 as a potential key regulator of muscle cachexia.
Background: Cancer cachexia is a progressive wasting syndrome and the most prevalent characteristic of cancer in patients with advanced pancreatic adenocarcinoma. We hypothesize that genes expressed in wasted skeletal muscle of pancreatic cancer patients may determine the initiation and severity of...
| Published in: | BMC Cancer Vol. 12; no. 1; pp. 265 - 266 |
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| Main Authors: | , , , , |
| Format: | research Journal Article |
| Published: |
BioMed Central
2012
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104374548&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104374548 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 14712407 1CH9 jtl: BMC Cancer issn: 14712407 maglogo: N pubinfo: dt: 2012 vid: 12 iid: 1 pid: 24147 pub: BioMed Central artinfo: ui: 104374548 NLM22721276 2011713459 10.1186/1471-2407-12-265 NLM22721276 PMC3465185 104374548 ppf: 265 ppct: 1 formats: tig: atl: Real-imaging cDNA-AFLP transcript profiling of pancreatic cancer patients: Egr-1 as a potential key regulator of muscle cachexia. aug: au: Skorokhod, Alexander Bachmann, Jeannine Giese, Nathalia A Martignoni, Marc E Krakowski-Roosen, Holger affil: Division of Preventive Oncology (G110), National Center for Tumor Diseases (NCT) Heidelberg, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 581, 69120, Heidelberg, Germany. Alexander.Skorokhod@med.uni-heidelberg.de. sug: subj: Cachexia Proteins Metabolism Gene Expression Profiling Muscle, Skeletal Pathology Pancreatic Neoplasms Pancreatic Neoplasms Pathology Aged Biopsy Proteins Female Genes Human Male Middle Age Muscle, Skeletal Metabolism Reproducibility of Results Aged: 65+ years Middle Aged: 45-64 years Female Male ab: Background: Cancer cachexia is a progressive wasting syndrome and the most prevalent characteristic of cancer in patients with advanced pancreatic adenocarcinoma. We hypothesize that genes expressed in wasted skeletal muscle of pancreatic cancer patients may determine the initiation and severity of cachexia syndrome.Experimental Design: We studied gene expression in skeletal muscle biopsies from pancreatic cancer patients with and without cachexia utilizing Real-Imaging cDNA-AFLP-based transcript profiling for genome-wide expression analysis.Results: Our approach yielded 183 cachexia-associated genes. Ontology analysis revealed characteristic changes for a number of genes involved in muscle contraction, actin cytoskeleton rearrangement, protein degradation, tissue hypoxia, immediate early response and acute-phase response.Conclusions: We demonstrate that Real-Imaging cDNA-AFLP analysis is a robust method for high-throughput gene expression studies of cancer cachexia syndrome in patients with pancreatic cancer. According to quantitative RT-PCR validation, the expression levels of genes encoding the acute-phase proteins α-antitrypsin and fibrinogen α and the immediate early response genes Egr-1 and IER-5 were significantly elevated in the skeletal muscle of wasted patients. By immunohistochemical and Western immunoblotting analysis it was shown, that Egr-1 expression is significantly increased in patients with cachexia and cancer. This provides new evidence that chronic activation of systemic inflammatory response might be a common and unifying factor of muscle cachexia. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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