Quantitative analysis of optical coherence tomography and histopathology images of normal and dysplastic oral mucosal tissues.
Selecting the most representative site for biopsy is crucial in establishing a definitive diagnosis of oral epithelial dysplasia. The current process involves clinical examination that can be subjective and prone to sampling errors. The aim of this study was therefore to investigate the use of optic...
| Publicado en: | Lasers in Medical Science Vol. 27; no. 4; pp. 795 - 805 |
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| Autores principales: | , , , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Springer Nature
Jul2012
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104382700&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104382700 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 02688921 O4P jtl: Lasers in Medical Science issn: 02688921 maglogo: N pubinfo: dt: Jul2012 vid: 27 iid: 4 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 104382700 NLM21850480 2011752888 10.1007/s10103-011-0975-1 NLM21850480 104382700 ppf: 795 ppct: 10 formats: tig: atl: Quantitative analysis of optical coherence tomography and histopathology images of normal and dysplastic oral mucosal tissues. aug: au: Adegun OK Tomlins PH Hagi-Pavli E McKenzie G Piper K Bader DL Fortune F Adegun, Oluyori Kutulola Tomlins, Pete H Hagi-Pavli, Eleni McKenzie, Gordon Piper, Kim Bader, Dan L Fortune, Farida affil: Barts & The London School of Medicine and Dentistry, Centre for Clinical and Diagnostic Oral Sciences, Institute of Dentistry, Queen Mary University of London, London, Whitechapel, UK sug: subj: Epithelium Pathology Mouth Mucosa Pathology Tomography Methods Biopsy Methods Epithelium Anatomy and Histology Human Diagnostic Imaging Mouth Diseases Pathology Mouth Mucosa Anatomy and Histology Polyps Pathology ab: Selecting the most representative site for biopsy is crucial in establishing a definitive diagnosis of oral epithelial dysplasia. The current process involves clinical examination that can be subjective and prone to sampling errors. The aim of this study was therefore to investigate the use of optical coherence tomography (OCT) for differentiation of normal and dysplastic oral epithelial samples, with a view to developing an objective and reproducible approach for biopsy site selection. Biopsy samples from patients with fibro-epithelial polyps (n = 13), mild dysplasia (n = 2), and moderate/severe dysplasia (n = 4) were scanned at 5-μm intervals using an OCT microscope and subsequently processed and stained with hematoxylin and eosin (H&E). Epithelial differentiation was measured from the rate of change (gradient) of the backscattered light intensity in the OCT signal as a function of depth. This parameter is directly related to the density of optical scattering from the cell nuclei. OCT images of normal oral epithelium showed a clear delineation of the mucosal layers observed in the matching histology. However, OCT images of oral dysplasia did not clearly identify the individual mucosal layers because of the increased density of abnormal cell nuclei, which impeded light penetration. Quantitative analysis on 2D-OCT and histology images differentiated dysplasia from normal control samples. Similar analysis on 3D-OCT datasets resulted in the reclassification of biopsy samples into the normal/mild and moderate/severe groups. Quantitative differentiation of normal and dysplastic lesions using OCT offers a non-invasive objective approach for localizing the most representative site to biopsy, particularly in oral lesions with similar clinical features. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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