Imaging the impact of Nox4 in cycling hypoxia-mediated U87 glioblastoma invasion and infiltration.
Purpose: We determined the impact of the cycling hypoxia tumor microenvironment on tumor cell invasion and infiltration in U87 human glioblastoma cells and investigated the underlying mechanisms using molecular bio-techniques and imaging.Procedures: The invasive phenotype of U87 cells and xenografts...
| Publicado en: | Molecular Imaging & Biology Vol. 14; no. 4; pp. 489 - 500 |
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| Autores principales: | , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Springer Nature
Aug2012
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104382890&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104382890 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15361632 KJU jtl: Molecular Imaging & Biology issn: 15361632 maglogo: N pubinfo: dt: Aug2012 vid: 14 iid: 4 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 104382890 NLM21870211 2011753433 10.1007/s11307-011-0516-0 NLM21870211 104382890 ppf: 489 ppct: 11 formats: fmt: @attributes: type: P tig: atl: Imaging the impact of Nox4 in cycling hypoxia-mediated U87 glioblastoma invasion and infiltration. aug: au: Hsieh CH Chang HT Shen WC Shyu WC Liu RS Hsieh, Chia-Hung Chang, Hao-Teng Shen, Wu-Chung Shyu, Woei-Cherng Liu, Ren-Shyan affil: Graduate Institute of Basic Medical Science, China Medical University and Hospital, Taichung, Taiwan sug: subj: Glioma Glioma Pathology Molecular Imaging Methods Oxidoreductases Metabolism Animal Studies Biochemical Phenomena Cell Movement Cell Physiology Cells Genetic Techniques Mice Neoplasm Invasiveness Peptide Hydrolases Metabolism Proteins Metabolism Reactive Oxygen Species Metabolism Transferases Metabolism ab: Purpose: We determined the impact of the cycling hypoxia tumor microenvironment on tumor cell invasion and infiltration in U87 human glioblastoma cells and investigated the underlying mechanisms using molecular bio-techniques and imaging.Procedures: The invasive phenotype of U87 cells and xenografts exposed to experimentally imposed cycling hypoxic stress in vitro and in vivo was determined by the matrigel invasion assay in vitro and dual optical reporter gene imaging in vivo. RNAi-knockdown technology was utilized to study the role of the NADPH oxidase subunit 4 (Nox4) on cycling hypoxia-mediated tumor invasion.Results: Cycling hypoxic stress significantly promoted tumor invasion in vitro and in vivo. However, Nox4 knockdown inhibited this effect. Nox4-generated reactive oxygen species (ROS) are required for cycling hypoxia-induced invasive potential in U87 cells through the activation of NF-κB- and ERK-mediated stimulation of MMP-9.Conclusions: Cycling hypoxia-induced ROS via Nox4 should be considered for therapeutic targeting of tumor cell invasion and infiltration in glioblastoma. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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