A multimode optical imaging system for preclinical applications in vivo: technology development, multiscale imaging, and chemotherapy assessment.

Purpose: Several established optical imaging approaches have been applied, usually in isolation, to preclinical studies; however, truly useful in vivo imaging may require a simultaneous combination of imaging modalities to examine dynamic characteristics of cells and tissues. We developed a new mult...

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Publicado en:Molecular Imaging & Biology Vol. 14; no. 4; pp. 431 - 443
Autores principales: Hwang JY, Wachsmann-Hogiu S, Ramanujan VK, Ljubimova J, Gross Z, Gray HB, Medina-Kauwe LK, Farkas DL, Hwang, Jae Youn, Wachsmann-Hogiu, Sebastian, Ramanujan, V Krishnan, Ljubimova, Julia, Gross, Zeev, Gray, Harry B, Medina-Kauwe, Lali K, Farkas, Daniel L
Formato: research Journal Article
Publicado: Springer Nature Aug2012
Acceso en línea:Ver este registro en EBSCOhost
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      jtl: Molecular Imaging & Biology
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      dt: Aug2012
      vid: 14
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      pub: Springer Nature
      place: New York, New York
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        atl: A multimode optical imaging system for preclinical applications in vivo: technology development, multiscale imaging, and chemotherapy assessment.
      aug:
        au:
          Hwang JY
          Wachsmann-Hogiu S
          Ramanujan VK
          Ljubimova J
          Gross Z
          Gray HB
          Medina-Kauwe LK
          Farkas DL
          Hwang, Jae Youn
          Wachsmann-Hogiu, Sebastian
          Ramanujan, V Krishnan
          Ljubimova, Julia
          Gross, Zeev
          Gray, Harry B
          Medina-Kauwe, Lali K
          Farkas, Daniel L
        affil: Minimally Invasive Surgical Technologies Institute and Department of Surgery, Cedars-Sinai Medical Center, 8700 Beverly Blvd. D6061, Los Angeles, CA 90048, USA
      sug:
        subj:
          Antineoplastic Agents Therapeutic Use
          Equipment and Supplies
          Molecular Imaging Methods
          Neoplasms Diagnosis
          Neoplasms Drug Therapy
          Xenografts
          Animal Studies
          Cells
          Human
          Immunity
          Luminescent Measurements
          Mice
          Nanotechnology
          Pilot Studies
          Rats
          Spectrometry, Fluorescence
      ab: Purpose: Several established optical imaging approaches have been applied, usually in isolation, to preclinical studies; however, truly useful in vivo imaging may require a simultaneous combination of imaging modalities to examine dynamic characteristics of cells and tissues. We developed a new multimode optical imaging system designed to be application-versatile, yielding high sensitivity, and specificity molecular imaging.Procedures: We integrated several optical imaging technologies, including fluorescence intensity, spectral, lifetime, intravital confocal, two-photon excitation, and bioluminescence, into a single system that enables functional multiscale imaging in animal models.Results: The approach offers a comprehensive imaging platform for kinetic, quantitative, and environmental analysis of highly relevant information, with micro-to-macroscopic resolution. Applied to small animals in vivo, this provides superior monitoring of processes of interest, represented here by chemo-/nanoconstruct therapy assessment.Conclusions: This new system is versatile and can be optimized for various applications, of which cancer detection and targeted treatment are emphasized here.
      pubtype: Academic Journal
      doctype:
        research
        Journal Article
      ougenre: Article
    language: English
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