Cryo-image analysis of tumor cell migration, invasion, and dispersal in a mouse xenograft model of human glioblastoma multiforme.
Purpose: The goals of this study were to create cryo-imaging methods to quantify characteristics (size, dispersal, and blood vessel density) of mouse orthotopic models of glioblastoma multiforme (GBM) and to enable studies of tumor biology, targeted imaging agents, and theranostic nanoparticles.Proc...
| Publicado en: | Molecular Imaging & Biology Vol. 14; no. 5; pp. 572 - 584 |
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| Autores principales: | , , , , , , , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Springer Nature
Oct2012
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104383294&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104383294 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15361632 KJU jtl: Molecular Imaging & Biology issn: 15361632 maglogo: N pubinfo: dt: Oct2012 vid: 14 iid: 5 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 104383294 NLM22125093 2011754223 10.1007/s11307-011-0525-z NLM22125093 PMC3444683 104383294 ppf: 572 ppct: 12 formats: fmt: @attributes: type: P tig: atl: Cryo-image analysis of tumor cell migration, invasion, and dispersal in a mouse xenograft model of human glioblastoma multiforme. aug: au: Qutaish MQ Sullivant KE Burden-Gulley SM Lu H Roy D Wang J Basilion JP Brady-Kalnay SM Wilson DL Qutaish, Mohammed Q Sullivant, Kristin E Burden-Gulley, Susan M Lu, Hong Roy, Debashish Wang, Jing Basilion, James P Brady-Kalnay, Susann M Wilson, David L affil: Department of Biomedical Engineering, Case Western Reserve University, Room 319 Wickenden Bldg., 2071 Martin Luther King Jr. Drive, Cleveland, OH 44106-7207, USA sug: subj: Brain Neoplasms Diagnosis Brain Neoplasms Pathology Cell Movement Glioma Diagnosis Glioma Pathology Image Processing, Computer Assisted Methods Algorithms Animal Studies Body Weights and Measures Brain Neoplasms Blood Supply Cells Cryopreservation Freezing Glioma Blood Supply In Vivo Studies Mice Neoplasm Invasiveness Neovascularization, Pathologic Pathology ab: Purpose: The goals of this study were to create cryo-imaging methods to quantify characteristics (size, dispersal, and blood vessel density) of mouse orthotopic models of glioblastoma multiforme (GBM) and to enable studies of tumor biology, targeted imaging agents, and theranostic nanoparticles.Procedures: Green fluorescent protein-labeled, human glioma LN-229 cells were implanted into mouse brain. At 20-38 days, cryo-imaging gave whole brain, 4-GB, 3D microscopic images of bright field anatomy, including vasculature, and fluorescent tumor. Image analysis/visualization methods were developed.Results: Vessel visualization and segmentation methods successfully enabled analyses. The main tumor mass volume, the number of dispersed clusters, the number of cells/cluster, and the percent dispersed volume all increase with age of the tumor. Histograms of dispersal distance give a mean and median of 63 and 56 μm, respectively, averaged over all brains. Dispersal distance tends to increase with age of the tumors. Dispersal tends to occur along blood vessels. Blood vessel density did not appear to increase in and around the tumor with this cell line.Conclusion: Cryo-imaging and software allow, for the first time, 3D, whole brain, microscopic characterization of a tumor from a particular cell line. LN-229 exhibits considerable dispersal along blood vessels, a characteristic of human tumors that limits treatment success. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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