Germinal center texture entropy as possible indicator of humoral immune response: immunophysiology viewpoint.
Purpose: In this article, we present the results indicating that spleen germinal center (GC) texture entropy determined by gray-level co-occurrence matrix (GLCM) method is related to humoral immune response.Procedures: Spleen tissue was obtained from eight outbred male short-haired guinea pigs previ...
| Publicado en: | Molecular Imaging & Biology Vol. 14; no. 5; pp. 534 - 541 |
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| Autores principales: | , , , |
| Formato: | research Journal Article |
| Publicado: |
Springer Nature
Oct2012
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104383298&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104383298 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15361632 KJU jtl: Molecular Imaging & Biology issn: 15361632 maglogo: N pubinfo: dt: Oct2012 vid: 14 iid: 5 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 104383298 NLM22160876 2011754225 10.1007/s11307-011-0531-1 NLM22160876 104383298 ppf: 534 ppct: 7 formats: fmt: @attributes: type: P tig: atl: Germinal center texture entropy as possible indicator of humoral immune response: immunophysiology viewpoint. aug: au: Pantic I Pantic S Pantic, Igor Pantic, Senka affil: Institute of Medical Physiology, Faculty of Medicine, University of Belgrade, Višegradska 26/II, 11000, Belgrade, Serbia sug: subj: Antibody Formation Immunology Image Processing, Computer Assisted Lymphoid Tissue Immunology Lymphoid Tissue Physiology Physics Animal Studies Guinea Pigs Lymphoid Tissue Male Sheep Male ab: Purpose: In this article, we present the results indicating that spleen germinal center (GC) texture entropy determined by gray-level co-occurrence matrix (GLCM) method is related to humoral immune response.Procedures: Spleen tissue was obtained from eight outbred male short-haired guinea pigs previously immunized by sheep red blood cells (SRBC). A total of 312 images from 39 germinal centers (156 GC light zone images and 156 GC dark zone images) were acquired and analyzed by GLCM method. Angular second moment, contrast, correlation, entropy, and inverse difference moment were calculated for each image. Humoral immune response to SRBC was measured using T cell-dependent antibody response (TDAR) assay.Results: Statistically highly significant negative correlation was detected between light zone entropy and the number of TDAR plaque-forming cells (r (s) = -0.86, p < 0.01). The entropy decreased as the plaque-forming cells increased and vice versa. A statistically significant negative correlation was also detected between dark zone entropy values and the number of plaque-forming cells (r (s) = -0.69, p < 0.05).Conclusions: Germinal center texture entropy may be a powerful indicator of humoral immune response. This study is one of the first to point out the potential scientific value of GLCM image texture analysis in lymphoid tissue cytoarchitecture evaluation. Lymphoid tissue texture analysis could become an important and affordable addition to the conventional immunophysiology techniques. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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