Time-dependent biological differences in molecular markers of high-grade urothelial cancer over 7 decades (ras proteins, pTEN, uPAR, PAI-1 and MMP-9).
The aim of this study was to evaluate changes and correlations between various molecular markers related to growth regulation and invasiveness in urothelial carcinomas in samples collected from 1932 to 2004. Paraffin-embedded autopsy/biopsy tissues from 144 patients were stained with antibodies agai...
| Publicado en: | Virchows Archiv: European Journal of Pathology Vol. 461; no. 5; pp. 541 - 552 |
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| Autores principales: | , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Springer Nature
Nov2012
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104383881&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104383881 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09456317 O1Z jtl: Virchows Archiv: European Journal of Pathology issn: 09456317 maglogo: N pubinfo: dt: Nov2012 vid: 461 iid: 5 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 104383881 NLM23052373 2011754915 10.1007/s00428-012-1323-y NLM23052373 104383881 ppf: 541 ppct: 11 formats: fmt: @attributes: type: P tig: atl: Time-dependent biological differences in molecular markers of high-grade urothelial cancer over 7 decades (ras proteins, pTEN, uPAR, PAI-1 and MMP-9). aug: au: Litlekalsoy J Hostmark JG Costea DE Illemann M Laerum OD Litlekalsoy, Jorunn Hostmark, Jens G Costea, Daniela Elena Illemann, Martin Laerum, Ole Didrik affil: The Gade Institute, Section for Pathology, University of Bergen, 5021 Bergen, Norway sug: subj: Carcinoma, Transitional Cell Metabolism Proteins Metabolism Tumor Markers, Biological Metabolism Urologic Neoplasms Metabolism Aged Carcinoma, Transitional Cell Pathology Female Human Immunohistochemistry In Situ Hybridization Male Peptide Hydrolases Metabolism Glycoproteins Metabolism Phosphatases Metabolism Blood Coagulation Factors Metabolism Receptors, Cell Surface Metabolism Time Factors Urologic Neoplasms Pathology Aged: 65+ years Female Male ab: The aim of this study was to evaluate changes and correlations between various molecular markers related to growth regulation and invasiveness in urothelial carcinomas in samples collected from 1932 to 2004. Paraffin-embedded autopsy/biopsy tissues from 144 patients were stained with antibodies against H-K-N ras proteins, pTEN protein, urokinase plasminogen activator receptor (uPAR), plasminogen activator inhibitor-1 (PAI-1) and matrix metalloproteinase-9 (MMP-9) and analyzed by in situ hybridization. Statistical analysis was performed by SPSS using cross tabulation and logistic regression. While the presence of K-ras, N-ras, PAI-1, and loss of pTEN increased over the last few decades, uPAR expression decreased during the same period. The increase in K-ras expression associated positively with the increase in expression of the other two ras proteins, H-ras and N-ras, and the loss of pTEN. A strong positive correlation was also observed between PAI-1 and uPAR, PAI-1 and previously detected markers, EGFR (epidermal growth factor receptor) and p53. Presence of uPAR was found to be positively associated with p16 expression. Multivariate analysis with clinical parameters revealed a positive correlation between PAI-1 expression and tumour grade, CkHMW (high molecular weight cytokeratin) and tumour grade, CkHMW and metastasis, EGFR and metastasis. mRNA could be detected in samples from the last 50 years while older samples were negative, indicating its complete degradation during longer storage. In conclusion, increased accumulation of K-ras, N-ras, and PAI-1 together with loss of pTEN in bladder carcinomas of grades II and III seems to be more dominant in recent times, suggesting an altered malignant potential in these neoplasms. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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