Effects of 5-Lipoxygenase Inhibitors on Guinea Pig Trachea in Ovalbumin-Induced Experimental Asthma.
Objective: We tested the hypothesis that embryonic neural stem cell (ENSC) therapy reduces neuronal DNA damage in the lesional spinal cord (SC) region and assessed the significance of some DNA breakdown products in urine and tissue samples as biomarkers of efficiency of the ENSC therapy. Material an...
| Publicado en: | Turkiye Klinikleri Journal of Medical Sciences Vol. 32; no. 5; pp. 1235 - 1241 |
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| Autores principales: | , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Turkiye Klinikleri
Oct2012
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104385407&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104385407 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 13000292 1VOP jtl: Turkiye Klinikleri Journal of Medical Sciences issn: 13000292 maglogo: N pubinfo: dt: Oct2012 vid: 32 iid: 5 pid: 67298 pub: Turkiye Klinikleri artinfo: ui: 104385407 2011758893 10.5336/medsci.2011-24877 104385407 ppf: 1235 ppct: 6 formats: fmt: @attributes: type: P tig: atl: Effects of 5-Lipoxygenase Inhibitors on Guinea Pig Trachea in Ovalbumin-Induced Experimental Asthma. aug: au: GÜREL, Ayse TIMLIOLU, Özenç affil: Department of Pharmacology, Fatih University Faculty of Medicine, Ankara sug: subj: Asthma Drug Therapy Enzyme Inhibitors Therapeutic Use Trachea Drug Effects Analysis of Variance Animal Studies Descriptive Statistics Guinea Pigs Kruskal-Wallis Test Models, Biological Post Hoc Analysis ab: Objective: We tested the hypothesis that embryonic neural stem cell (ENSC) therapy reduces neuronal DNA damage in the lesional spinal cord (SC) region and assessed the significance of some DNA breakdown products in urine and tissue samples as biomarkers of efficiency of the ENSC therapy. Material and Methods: DNA damage was measured by single cell gel electrophoresis (SCGE/Comet Assay) in spinal cord tissue of rats 7 (acute group) and 28 (chronic group) days after surgical resections of a 10 mm segment of the cord below T8-T9 and sham operations. As biomarkers of DNA damage, urinary 5-(hydroxymethyl) uracil (5HMU) and 2\'-deoxyuridine (2dU) were analyzed by high-performance liquid chromatography (HPLC) with electrochemical detection (ED). Results: Comet scores of the lesioned tissues were significantly higher in the acute and chronic spinal cord injury (SCI) compared to the sham operated groups (p=0.009 and p=0.009, respectively). After the ENSC transplantation, tail %, tail length and tail moment (T%, TL and TM, respectively) decreased in acute (p=0.009, p=0.009 and p=0.009, respectively) and chronically (p=0.009, p=0.009, p=0.009 respectively) injured rats. 5HMU and 2dU were significantly higher in the acute and the chronic groups than in the sham operated groups (p=0.009 and p=0.009, respectively). 5HMU concentration in the acute SCI group had a stronger correlation strongly with tail moment (r=0.9039, p=0.02). In the chronic injury group, 5HMU and 2dU concentrations correlated significantly with tail length (r=0.9990, p=0.001 and r=0.9272, p=0.016, respectively). While 5HMU seems to be a better biomarker for acute injury, both HMU and 2dU excretions increase in chronic damage. Besides, neurological functions of all the study animals were measured with the Basso-Beattie-Bresnahan (BBB) score. Conclusion: Our results demonstrated that ENSC therapy of the lesioned tissue significantly decreased the levels of DNA breakdown products in urine and tissue samples of rats with acute and chronic SCI. Assessment of neurological recovery with the BBB test confirmed these findings. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: Turkish refInfo: holdings: @attributes: islocal: N |
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