Local Angioneogenetic Effect of Intramuscular Interleukin-8 Injection.
Objective: Angioneogenesis, which plays significant roles in a variety of physiological processes such as embryonic growth and wound healing, is strictly delimited and is finely tuned by a balance of proangiogenic and antiangiogenic factors. This study was conducted to investigate the angioneogenic...
| Publicado en: | Turkiye Klinikleri Journal of Medical Sciences Vol. 32; no. 5; pp. 1273 - 1278 |
|---|---|
| Autores principales: | , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Turkiye Klinikleri
Oct2012
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104385413&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104385413 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 13000292 1VOP jtl: Turkiye Klinikleri Journal of Medical Sciences issn: 13000292 maglogo: N pubinfo: dt: Oct2012 vid: 32 iid: 5 pid: 67298 pub: Turkiye Klinikleri artinfo: ui: 104385413 2011758899 10.5336/medsci.2011-26065 104385413 ppf: 1273 ppct: 5 formats: fmt: @attributes: type: P tig: atl: Local Angioneogenetic Effect of Intramuscular Interleukin-8 Injection. aug: au: BOZOK, Sahin YÜREKLI, Ismail AKSUN, Murat ORTAÇ, Ragip TULUKOLU, Engin SAHIN, Aykut affil: Department of Cardiovascular Surgery, Rize University Faculty of Medicine, Rize sug: subj: Injections, Intramuscular Interleukins Administration and Dosage Neovascularization, Physiologic Animal Studies Data Analysis Software Descriptive Statistics Kruskal-Wallis Test Mann-Whitney U Test Models, Biological Rabbits Staining and Labeling ab: Objective: Angioneogenesis, which plays significant roles in a variety of physiological processes such as embryonic growth and wound healing, is strictly delimited and is finely tuned by a balance of proangiogenic and antiangiogenic factors. This study was conducted to investigate the angioneogenic effect of interleukin-8 (IL-8) administered intramuscularly. Material and Methods: Twelve New Zealand white rabbits were included in this study. A total daily dose of 4 micrograms (1 mcg/kg) of IL-8 was administered into the left (Group A) and saline solution into the right (Group B) gluteus maximus muscles of 6 rabbits for 6 days. The remaining 6 rabbits constituted the sham group (Group C). Gluteal muscle samples were obtained from injection sites in all groups and were stained with hematoxylin-eosin and immunohistochemically by using streptavidin biotin method with CD31 antibody. Avidin-biotin peroxidase method (LSAB) was used as secondary and binding antibody. Diaminobenzidine tetrahydrochloride (DAB) was used as chromogenic substance. In immunohistochemical staining with CD31, vascular channels covered with brown stained cells or cell clusters were considered and were counted as vascular network. Results: Three subjects in Group A and one subject in Group B displayed findings of large muscle necrosis and regeneration. Vascular channel score was significantly higher in Group A (p=0.032) (Group A; median=12.5, min=6, max=16. Group B; median=5, min=4, max=13. Group C; median=4.5, min=4, max=13.) than in the other groups. Conclusion: IL-8 chemokine family seems to stimulate angioneogenesis in rabbit skeletal muscles. This result is promising in terms of the possible therapeutic potential of IL-8. Daily administration at a dose of around 1 mcg/kg caused local tissue necrosis, hence use of alternative routes such as intraarterial administration must be investigated to avoid such complications. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: Turkish refInfo: holdings: @attributes: islocal: N |
|---|