Visualising liver fibrosis by phase-contrast X-ray imaging in common bile duct ligated mice.
Objectives: To determine whether phase-contrast X-ray imaging can be used to visualise directly the accumulated extracellular matrix proteins associated with liver fibrosis in common bile duct ligated mice.Methods: Twenty-six-week-old C57BL female mice were randomised into three groups. In groups 1...
| Publicado en: | European Radiology Vol. 23; no. 2; pp. 417 - 424 |
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| Autores principales: | , , , , , , , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Springer Nature
Feb2013
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104413609&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104413609 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09387994 NPH jtl: European Radiology issn: 09387994 maglogo: N pubinfo: dt: Feb2013 vid: 23 iid: 2 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 104413609 NLM22903640 2011893890 10.1007/s00330-012-2630-z NLM22903640 104413609 ppf: 417 ppct: 7 formats: fmt: @attributes: type: P tig: atl: Visualising liver fibrosis by phase-contrast X-ray imaging in common bile duct ligated mice. aug: au: Zhang X Yang XR Chen Y Li HQ Li RM Yuan QX Zhu PP Huang WX Peng WJ Zhang, Xi Yang, Xin-Rong Chen, Yu Li, Hai-Qing Li, Rui-Min Yuan, Qing-Xi Zhu, Pei-Ping Huang, Wan-Xia Peng, Wei-Jun affil: Department of Radiology, Affiliated Cancer Hospital of Fudan University, 270 Dongan Road, Shanghai 200032, China sug: subj: Contrast Media Diagnostic Use Liver Cirrhosis Pathology Liver Cirrhosis Radiography Radiography Methods Animal Studies Biliary Tract Surgical Procedures Biopsy, Needle Female Immunohistochemistry Ligation Mice Models, Biological Random Assignment Reference Values Sensitivity and Specificity Surgery, Digestive System Methods Tissue Culture Techniques Female ab: Objectives: To determine whether phase-contrast X-ray imaging can be used to visualise directly the accumulated extracellular matrix proteins associated with liver fibrosis in common bile duct ligated mice.Methods: Twenty-six-week-old C57BL female mice were randomised into three groups. In groups 1 (n = 5) and 2 (n = 10), common bile duct ligation was conducted to produce secondary biliary cirrhosis. Mouse livers were then excised 15 (group 1) and 40 days (group 2) after the ligation of the common bile duct for imaging. In the control group, the livers of 5 mice were excised 40 days after the sham operation. Images were then acquired using the analyser crystal set at different positions of the rocking curve.Results: The results show that the fibrotic septa and hepatic lobules enclosed by fibrotic septa can be visualised clearly at the whole organ level via phase-contrast X-ray imaging without any contrast agent.Conclusion: These results suggest that phase-contrast X-ray imaging can easily reveal the accumulated extracellular matrix proteins associated with liver fibrosis without using any contrast agent and has great potential in the study of liver fibrosis. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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