Expressional Analyses of NM23-H1, KAI1and MKK4 Metastasis-Related Genes in Metastatic Ovarian Carcinomas.
Objective: Cytoreductive surgery as a basis of therapy in epithelial ovarian carcinomas (EOC) provides the primary tumor and metastatic tumor samples from a same patient. This gives an excellent opportunity for evaluation of metastatic factors by excluding inter-individual differences. Therefore, we...
| Published in: | Turkiye Klinikleri Journal of Medical Sciences Vol. 32; no. 4; pp. 984 - 990 |
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| Main Authors: | , , , , , , |
| Format: | pictorial research tables/charts Journal Article |
| Published: |
Turkiye Klinikleri
Aug2012
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104415884&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104415884 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 13000292 1VOP jtl: Turkiye Klinikleri Journal of Medical Sciences issn: 13000292 maglogo: N pubinfo: dt: Aug2012 vid: 32 iid: 4 pid: 67298 pub: Turkiye Klinikleri artinfo: ui: 104415884 2011681425 10.5336/medsci.2011-26006 104415884 ppf: 984 ppct: 6 formats: fmt: @attributes: type: P tig: atl: Expressional Analyses of NM23-H1, KAI1and MKK4 Metastasis-Related Genes in Metastatic Ovarian Carcinomas. aug: au: Bilgen, Türker Erdogan, Gülgün Simsek, Tayup Gülkesen, Hakan Pestereli, Elif Karaveli, Seyda Keser, Ibrahim affil: Department of Medical Biology, Akdeniz University Faculty of Medicine, Antalya sug: subj: Gene Expression Neoplasm Metastasis Familial and Genetic Ovarian Neoplasms Complications Academic Medical Centers Data Analysis Software Female Funding Source Human Reverse Transcriptase Polymerase Chain Reaction RNA Analysis Turkiye Wilcoxon Signed Rank Test Female ab: Objective: Cytoreductive surgery as a basis of therapy in epithelial ovarian carcinomas (EOC) provides the primary tumor and metastatic tumor samples from a same patient. This gives an excellent opportunity for evaluation of metastatic factors by excluding inter-individual differences. Therefore, we aimed to define changes at mRNA levels of NM23-H1, KAI1 and MKK4 metastasis-related genes in the paired normal tissue, primary tumor and omental metastatic tumor samples obtained from a same patient. Material and Methods: mRNA levels were quantified by quantitative reverse transcription polymerase chain reaction (Q-RT-PCR) following total RNA extraction in normal tissues, primary malign tissues of EOC, and its metastatic lesions on omentum for 41 patients with stage III-C (FIGO) EOC. Results: We found that mRNA level of NM23-H1 was significantly higher in metastatic samples compared to primary tumors (p=0.009). On the other hand, MKK4 was found to be significantly lower in primary tumor samples compared to normal tissues (p=0.024). There was no significant change at mRNA level of KAI1 among normal tissues, primary tumors and omental metastatic tumor samples. Conclusion: We suppose that in detailed functional studies, approaches that suppress NM23-H1 gene and restore MKK4 gene would make these genes important molecular targets for treatment of metastatic EOC in the future. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: Turkish refInfo: holdings: @attributes: islocal: N |
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