Angiogenin expression in burn blister fluid: Implications for its role in burn wound neovascularization.
Deep partial thickness burn ( DPTB) wound fluids have a greater propensity for establishing neovascularization than did superficial partial thickness burn ( SPTB) wound fluids in our previous study. To investigate the factors responsible for this activity, cytokine array and enzyme-linked immunosorb...
| Publicado en: | Wound Repair & Regeneration Vol. 20; no. 5; pp. 731 - 740 |
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| Autores principales: | , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
Sep/Oct2012
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104417730&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104417730 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10671927 DPV jtl: Wound Repair & Regeneration issn: 10671927 maglogo: Y pubinfo: dt: Sep/Oct2012 vid: 20 iid: 5 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 104417730 80026314 10.1111/j.1524-475X.2012.00819.x NLM22823522 104417730 ppf: 731 ppct: 9 formats: fmt: @attributes: type: P tig: atl: Angiogenin expression in burn blister fluid: Implications for its role in burn wound neovascularization. aug: au: Pan, Shin-Chen Wu, Li-Wha Chen, Chung-Lin Shieh, Shyh-Jou Chiu, Haw-Yen affil: Institute of Clinical Medicine, College of Medicine, National Cheng Kung University; Department of Surgery, Section of Plastic and Reconstructive Surgery, National Cheng Kung University Medical College and Hospital sug: subj: Burns Physiopathology Fluids and Secretions Neovascularization, Physiologic Human Funding Source Immunohistochemistry Cytokines Analysis Enzyme-Linked Immunosorbent Assay T-Tests Pearson's Correlation Coefficient Analysis of Variance ab: Deep partial thickness burn ( DPTB) wound fluids have a greater propensity for establishing neovascularization than did superficial partial thickness burn ( SPTB) wound fluids in our previous study. To investigate the factors responsible for this activity, cytokine array and enzyme-linked immunosorbent assay were used to perform an expression analysis of angiogenic factors in burn fluid. Although present in approximately equal amounts in both SPTB and DPTB blister fluids from burn patients, angiogenin does appear to be involved in the ability of DPTB blister fluid to promote neovascularization in vitro and in vivo. Angiogenin alone was sufficient to induce endothelial differentiation of circulating angiogenic cells ( CAC) without vascular endothelial growth factor A involvement. In addition, angiogenin was positively associated with CAC differentiation in the burn blister fluid. Blocking the effect of angiogenin in burn blister fluids resulted in a significant reduction of endothelial cell proliferation, CAC differentiation, and new blood vessels formation in vivo. Moreover, immunohistochemistry revealed that high angiogenin expression colocalizes with high vascularity in human burn wounds at day 7, further supporting our hypothesis that angiogenin is involved in burn wound neovascularization. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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