Evaluation of methods for sizing and counting of ultrasound contrast agents.
A precise, accurate and well documented method for the sizing and counting of microbubbles is essential for all aspects of quantitative microbubble-enhanced ultrasound imaging. The efficacy of (a) electro-impedance volumetric zone sensing (ES) also called a Coulter counter/multisizer; (b) optical mi...
| Publicado en: | Ultrasound in Medicine & Biology Vol. 38; no. 5; pp. 834 - 846 |
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| Autores principales: | , , , , , , , , , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Elsevier B.V.
May2012
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104435685&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104435685 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 03015629 JJ6 jtl: Ultrasound in Medicine & Biology issn: 03015629 maglogo: N pubinfo: dt: May2012 vid: 38 iid: 5 pid: 467 pub: Elsevier B.V. place: New York, New York artinfo: ui: 104435685 NLM22402020 2011733940 10.1016/j.ultrasmedbio.2012.01.012 NLM22402020 PMC3657178 104435685 ppf: 834 ppct: 12 formats: tig: atl: Evaluation of methods for sizing and counting of ultrasound contrast agents. aug: au: Sennoga CA Yeh JS Alter J Stride E Nihoyannopoulos P Seddon JM Haskard DO Hajnal JV Tang MX Eckersley RJ Sennoga, Charles A Yeh, James S M Alter, Julia Stride, Eleanor Nihoyannopoulos, Petros Seddon, John M Haskard, Dorian O Hajnal, Joseph V Tang, Meng-Xing Eckersley, Robert J affil: Imaging Sciences Department, Imperial College London, Hammersmith Campus, London, United Kingdom sug: subj: Drug Evaluation, Preclinical Methods Phospholipids Phospholipids Analysis Sulfur Compounds Sulfur Compounds Analysis Titrimetry Methods Ultrasonography Contrast Media Analysis Particle Size ab: A precise, accurate and well documented method for the sizing and counting of microbubbles is essential for all aspects of quantitative microbubble-enhanced ultrasound imaging. The efficacy of (a) electro-impedance volumetric zone sensing (ES) also called a Coulter counter/multisizer; (b) optical microscopy (OM); and (c) laser diffraction (LD), for the sizing and counting of microbubbles was assessed. Microspheres with certified mean diameter and number concentration were used to assess sizing and counting reproducibility (precision) and reliability (accuracy) of ES, OM and LD. SonoVue™ was repeatedly (n = 3) sized and counted to validate ES, OM and LD sizing and counting efficacy. Statistical analyses of intra-method variability for the SonoVue™ mean diameter showed that the best microbubble sizing reproducibility was obtained using OM with a mean diameter sizing variability of 1.1%, compared with a variability of 4.3% for ES and 7.1% for LD. The best microbubble counting reproducibility was obtained using ES with a number concentration variability of 8.3%, compared with a variability of 22.4% for OM and 32% for LD. This study showed that no method is fully suited to both sizing and counting of microbubbles. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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