Effects of Tepary Bean ( Phaseolus acutifolius ) Protease Inhibitor and Semipure Lectin Fractions on Cancer Cells.
Some natural and synthetic protease inhibitors (PI), such as the Bowman-Birk PI from soybean, have anticancer effects. We previously purified and characterized a Bowman-Birk-type PI from Tepary bean (Phaseolus acutifolius) seeds (TBPI). A semipure protein fraction containing this inhibitor, when tes...
| Published in: | Nutrition & Cancer Vol. 64; no. 8; pp. 1269 - 1279 |
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| Main Authors: | , , , , , , , , , |
| Format: | research tables/charts Journal Article |
| Published: |
Taylor & Francis Ltd
Nov2012
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104441692&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104441692 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01635581 7MS jtl: Nutrition & Cancer issn: 01635581 maglogo: N pubinfo: dt: Nov2012 vid: 64 iid: 8 pid: 377 pub: Taylor & Francis Ltd place: Philadelphia, Pennsylvania artinfo: ui: 104441692 83564597 10.1080/01635581.2012.722246 104441692 ppf: 1269 ppct: 10 formats: fmt: @attributes: type: P tig: atl: Effects of Tepary Bean ( Phaseolus acutifolius ) Protease Inhibitor and Semipure Lectin Fractions on Cancer Cells. aug: au: García-Gasca, Teresa García-Cruz, Marlen Hernandez-Rivera, Elisa López-Matínez, Josue Castañeda-Cuevas, Ana L. Yllescas-Gasca, Lorena Rodríguez-Méndez, Adriana J. Mendiola-Olaya, Elizabeth Castro-Guillén, José L. Blanco-Labra, Alejandro affil: Facultad de Ciencias Naturales. Universidad Autónoma de Querétaro, Querétaro, México sug: subj: Neoplasms Physiopathology Plants, Medicinal Therapeutic Use Protease Inhibitors Proteins Therapeutic Use Cell Physiology Nutrition Neoplasms Prevention and Control Biological Assay Classification Metalloproteins Data Analysis Data Analysis Software Post Hoc Analysis ab: Some natural and synthetic protease inhibitors (PI), such as the Bowman-Birk PI from soybean, have anticancer effects. We previously purified and characterized a Bowman-Birk-type PI from Tepary bean (Phaseolus acutifolius) seeds (TBPI). A semipure protein fraction containing this inhibitor, when tested its in vitro effect on transformed cells, showed a differential cytotoxic effect, as well as an increase in cell attachment to culture dishes. In this article we report that lectins were responsible for the cytotoxic effect previously observed, exhibiting a differential, antiproliferative effect on nontransformed cells and on different lineages of cancer cells. Although the purified TBPI lacked cytotoxicity, it was found to be responsible for the increase in cell adhesion, decreasing culture dishes’ extracellular matrix degradation, leading to a decrease of the in vitro cell invasion capacity. This effect coincided with the suppression of Matrix Metalloproteinase-9 activity. These results indicate that Tepary bean seeds contain at least 2 different groups of bioactive proteins with distinct effects on cancer cells. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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