Correlation of Serology with Morphological Changes in Gastric Biopsy in Helicobacter Pylori Infection and Evaluation of Immunohistochemistry for H. Pylori Identification.
Background/Aim: Helicobacter pylori is implicated in various gastroduodenal diseases and many tests are available for its detection. The present study attempted to document the morphological changes in the gastric mucosa induced by H. pylori colonization and correlate them with the severity of the i...
| Publicado en: | Saudi Journal of Gastroenterology Vol. 18; no. 6; pp. 369 - 375 |
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| Autores principales: | , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wolters Kluwer India Pvt Ltd
Nov2012
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104442354&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104442354 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 13193767 322P jtl: Saudi Journal of Gastroenterology issn: 13193767 maglogo: N pubinfo: dt: Nov2012 vid: 18 iid: 6 pid: 16919 pub: Wolters Kluwer India Pvt Ltd artinfo: ui: 104442354 83622307 10.4103/1319-3767.103428 104442354 ppf: 369 ppct: 6 formats: tig: atl: Correlation of Serology with Morphological Changes in Gastric Biopsy in Helicobacter Pylori Infection and Evaluation of Immunohistochemistry for H. Pylori Identification. aug: au: Shukla, Shailaja Pujani, Mukta Agarwal, Aditya Pujani, Meenu Rohtagi, Anurag affil: Department of Pathology, Medical College and Smt. Sucheta Kriplani Hospital, New Delhi, India sug: subj: Helicobacter Infections Diagnosis Stomach Endoscopy Methods Human Serology Utilization Biopsy Utilization Immunohistochemistry Utilization Severity of Illness Enzyme-Linked Immunosorbent Assay Utilization Equipment and Supplies Utilization Adult Middle Age Aged Male Female Stomach Pathology Sensitivity and Specificity Descriptive Statistics Antibodies Adult: 19-44 years Middle Aged: 45-64 years Aged: 65+ years Male Female ab: Background/Aim: Helicobacter pylori is implicated in various gastroduodenal diseases and many tests are available for its detection. The present study attempted to document the morphological changes in the gastric mucosa induced by H. pylori colonization and correlate them with the severity of the infection. The study also compared various diagnostic tests and evaluated the different staining methods used for H. pylori detection, especially immunohistochemical identification. Patients and Methods: One hundred and two patients with dyspepsia were included. Enzyme-linked immunosorbent assay (ELISA) for H. pylori-specific immunoglobulin G (IgG), immunoglobulin A (IgA), and immunoglobulin M (IgM) was used. Rapid urease test was performed on endoscopic biopsy and it was stained with hematoxylin and eosin (H and E), modified Giemsa, and immunohistochemical stains. Results: A significant correlation was found between the density of H. pylori and severity of gastritis. A significant correlation was observed between serology (especially when used in combination, IgG and IgA) and status of H. pylori. Immunohistochemical staining enhanced the diagnostic yield of H. pylori detection. Conclusions: Immunohistochemistry (IHC) should be used judiciously, whereas simple and economical tests like modified Giemsa should be used routinely for the detection of H. pylori. Combined ELISA (IgG and IgA) should be preferred over single ELISA. Simultaneous morphological and serological detection of H. pylori is preferable as H. pylori may not be detected on morphology alone due to its patchy distribution in the stomach. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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