Microvessel density but not neoangiogenesis is associated with 18F-FDG uptake in human atherosclerotic carotid plaques.

Introduction: The vulnerable atherosclerotic lesion exhibits the proliferation of neovessels and inflammation. The imaging modality 2-deoxy-2-[(18)F]fluoro-D: -glucose positron emission tomography ((18)FDG-PET) is considered for the identification of vulnerable plaques.Purpose: The purpose of this s...

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Published in:Molecular Imaging & Biology Vol. 14; no. 3; pp. 384 - 393
Main Authors: Pedersen SF, Graebe M, Hag AM, Hoejgaard L, Sillesen H, Kjaer A, Pedersen, Sune Folke, Graebe, Martin, Hag, Anne Mette Fisker, Hoejgaard, Liselotte, Sillesen, Henrik, Kjaer, Andreas
Format: research Journal Article
Published: Springer Nature Jun2012
Online Access:View this record in EBSCOhost
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      dt: Jun2012
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      pub: Springer Nature
      place: New York, New York
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        10.1007/s11307-011-0507-1
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        atl: Microvessel density but not neoangiogenesis is associated with 18F-FDG uptake in human atherosclerotic carotid plaques.
      aug:
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          Pedersen SF
          Graebe M
          Hag AM
          Hoejgaard L
          Sillesen H
          Kjaer A
          Pedersen, Sune Folke
          Graebe, Martin
          Hag, Anne Mette Fisker
          Hoejgaard, Liselotte
          Sillesen, Henrik
          Kjaer, Andreas
        affil: Cluster for Molecular Imaging, University of Copenhagen, Blegdamsvej 3, 2200, Copenhagen, Denmark
      sug:
        subj:
          Carotid Stenosis Metabolism
          Fludeoxyglucose F 18 Pharmacokinetics
          Blood Vessels Metabolism
          Neovascularization, Pathologic Metabolism
          Atherosclerosis Metabolism
          Aged
          Aged, 80 and Over
          Antigens, Surface
          Antigens, Surface Metabolism
          Carotid Stenosis Radiography
          Female
          Gene Expression Profiling
          Human
          Receptors, Cell Surface
          Receptors, Cell Surface Metabolism
          Linear Regression
          Male
          Blood Vessels Radiography
          Middle Age
          Neovascularization, Pathologic Radiography
          Atherosclerosis Radiography
          Tomography, Emission-Computed
          Endothelial Growth Factors
          Endothelial Growth Factors Metabolism
          Aged: 65+ years
          Aged, 80 & over
          Middle Aged: 45-64 years
          Female
          Male
      ab: Introduction: The vulnerable atherosclerotic lesion exhibits the proliferation of neovessels and inflammation. The imaging modality 2-deoxy-2-[(18)F]fluoro-D: -glucose positron emission tomography ((18)FDG-PET) is considered for the identification of vulnerable plaques.Purpose: The purpose of this study was to compare the gene expression of neoangiogenesis and vulnerability-associated genes with (18)FDG uptake in patients undergoing carotid endarterectomy.Procedures: Human atherosclerotic carotid artery plaques from symptomatic patients were used for gene expression analysis by quantitative PCR of vascular endothelial growth factor (VEGF) and integrin α(V) and integrin β(3) subunits, genes essential during neoangiogenesis. We also evaluated the gene expression of CD34, a measure of microvessel density (MVD), as well as CD68, MMP-9, and cathepsin K, genes of major importance in plaque vulnerability. Gene expression analysis was compared with (18)FDG-PET.Results: VEGF and integrin α(V)β(3) gene expression did not correlate with (18)FDG uptake, whereas CD34 gene expression exhibited an inverse correlation with (18)FDG uptake. Additionally, we established that markers of vulnerability were correlated with (18)FDG uptake.Conclusions: Neoangiogenesis is not associated with (18)FDG uptake, whereas MVD and markers of vulnerability correlate with (18)FDG uptake. The absence of correlation between markers of neoangiogenesis and (18)FDG uptake suggests a temporal separation between the process of neoangiogenesis and inflammatory activity.
      pubtype: Academic Journal
      doctype:
        research
        Journal Article
      ougenre: Article
    language: English
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