Quantification of skeletal blood flow and fluoride metabolism in rats using PET in a pre-clinical stress fracture model.
Purpose: Blood flow is an important factor in bone production and repair, but its role in osteogenesis induced by mechanical loading is unknown. Here, we present techniques for evaluating blood flow and fluoride metabolism in a pre-clinical stress fracture model of osteogenesis in rats.Procedures: B...
| Publicado en: | Molecular Imaging & Biology Vol. 14; no. 3; pp. 348 - 355 |
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| Autores principales: | , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Springer Nature
Jun2012
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104443622&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104443622 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15361632 KJU jtl: Molecular Imaging & Biology issn: 15361632 maglogo: N pubinfo: dt: Jun2012 vid: 14 iid: 3 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 104443622 NLM21785919 2011752284 10.1007/s11307-011-0505-3 NLM21785919 PMC3593647 104443622 ppf: 348 ppct: 7 formats: fmt: @attributes: type: P tig: atl: Quantification of skeletal blood flow and fluoride metabolism in rats using PET in a pre-clinical stress fracture model. aug: au: Tomlinson RE Silva MJ Shoghi KI Tomlinson, Ryan E Silva, Matthew J Shoghi, Kooresh I affil: Department of Orthopaedic Surgery, Washington University in St. Louis, St. Louis, MO 63110, USA sug: subj: Arm Bones Blood Supply Arm Bones Metabolism Fluorine Radioisotopes Pharmacokinetics Fractures, Stress Metabolism Fractures, Stress Radiography Tomography, Emission-Computed Methods Algorithms Animal Studies Arm Bones Injuries Arm Bones Radiography Kinetics Male Osteogenesis Physiology Radioisotopes Pharmacokinetics Rats Stress, Mechanical Male ab: Purpose: Blood flow is an important factor in bone production and repair, but its role in osteogenesis induced by mechanical loading is unknown. Here, we present techniques for evaluating blood flow and fluoride metabolism in a pre-clinical stress fracture model of osteogenesis in rats.Procedures: Bone formation was induced by forelimb compression in adult rats. (15)O water and (18)F fluoride PET imaging were used to evaluate blood flow and fluoride kinetics 7 days after loading. (15)O water was modeled using a one-compartment, two-parameter model, while a two-compartment, three-parameter model was used to model (18)F fluoride. Input functions were created from the heart, and a stochastic search algorithm was implemented to provide initial parameter values in conjunction with a Levenberg-Marquardt optimization algorithm.Results: Loaded limbs are shown to have a 26% increase in blood flow rate, 113% increase in fluoride flow rate, 133% increase in fluoride flux, and 13% increase in fluoride incorporation into bone as compared to non-loaded limbs (p < 0.05 for all results).Conclusions: The results shown here are consistent with previous studies, confirming this technique is suitable for evaluating the vascular response and mineral kinetics of osteogenic mechanical loading. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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