Loss of heterozygosity on chromosome 16q suggests malignancy in core needle biopsy specimens of intraductal papillary breast lesions.
It is often difficult to make a definitive diagnosis of papillary breast lesions using core needle biopsy (CNB) specimens. We studied loss of heterozygosity (LOH) on chromosome 16q in order to assess its diagnostic use for papillary breast lesions in CNB specimens. Of 25 patients with intraductal pa...
| Publicado en: | Virchows Archiv: European Journal of Pathology Vol. 460; no. 5; pp. 497 - 505 |
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| Autores principales: | , , , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Springer Nature
May2012
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104443749&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104443749 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09456317 O1Z jtl: Virchows Archiv: European Journal of Pathology issn: 09456317 maglogo: N pubinfo: dt: May2012 vid: 460 iid: 5 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 104443749 NLM22476400 2011752417 10.1007/s00428-012-1200-8 NLM22476400 104443749 ppf: 497 ppct: 8 formats: fmt: @attributes: type: P tig: atl: Loss of heterozygosity on chromosome 16q suggests malignancy in core needle biopsy specimens of intraductal papillary breast lesions. aug: au: Yoshida M Tsuda H Yamamoto S Kinoshita T Akashi-Tanaka S Hojo T Fukutomi T Yoshida, Miwa Tsuda, Hitoshi Yamamoto, Sohei Kinoshita, Takayuki Akashi-Tanaka, Sadako Hojo, Takashi Fukutomi, Takashi affil: Division of Diagnostic Pathology, National Cancer Center Hospital, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan sug: subj: Breast Neoplasms Carcinoma, Ductal, Breast Carcinoma, Papillary Chromosomes Neoplasms, Ductal, Lobular, and Medullary Biopsy, Needle Breast Neoplasms Pathology Carcinoma, Ductal, Breast Pathology Carcinoma, Papillary Pathology Diagnosis, Differential Female Mutation Neoplasms, Ductal, Lobular, and Medullary Pathology Reverse Transcriptase Polymerase Chain Reaction Female ab: It is often difficult to make a definitive diagnosis of papillary breast lesions using core needle biopsy (CNB) specimens. We studied loss of heterozygosity (LOH) on chromosome 16q in order to assess its diagnostic use for papillary breast lesions in CNB specimens. Of 25 patients with intraductal papillary breast tumors, we extracted DNA from paired samples of tumor cells from CNB specimens and non-tumor cells from subsequent excision specimens and analyzed LOH at the D16S419 and D16S514 loci on chromosome 16q. LOH analysis results were compared with final diagnoses based on pathological features of the resected specimens. On the CNB specimens, 21 tumors were histologically diagnosed as indeterminate or suspicious for malignancy, while four tumors were unambiguously malignant. Of the 21 indeterminate or suspicious tumors, 11 were finally diagnosed as benign and ten as malignant, and on these, LOH analyses were informative for 8 of the 11 benign tumors and 7 of the 10 malignant tumors. LOH was also informative on two of the four tumors unambiguously malignant on CNB. None of the eight informative benign tumors showed LOH on 16q. Six of the eleven informative malignant tumors showed LOH on 16q. LOH on 16q was significantly different between CNB specimens of benign and malignant intraductal papillary tumors (P = 0.007). Analysis of LOH on 16q may be helpful in making a definitive diagnosis in cases of papillary breast lesions, in both excised and CNB specimens. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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