Quantification of PML/RARa transcript after induction predicts outcome in children with acute promyelocytic leukemia.
Reports on the use of the real-time quantitative polymerase chain reaction (RQ-PCR) for childhood acute promyelocytic leukemia (APL) therapy are scarce. This study describes the prognostic significance of quantification of the PML-RARa transcript in children with APL. Since January 2004, we have ana...
| Publicado en: | International Journal of Hematology Vol. 95; no. 5; pp. 500 - 509 |
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| Autores principales: | , , , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Springer Nature
May2012
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104443779&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104443779 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09255710 3LY jtl: International Journal of Hematology issn: 09255710 maglogo: N pubinfo: dt: May2012 vid: 95 iid: 5 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 104443779 NLM22407853 2011752489 10.1007/s12185-012-1034-9 NLM22407853 104443779 ppf: 500 ppct: 9 formats: tig: atl: Quantification of PML/RARa transcript after induction predicts outcome in children with acute promyelocytic leukemia. aug: au: Zhang L Cao Z Zou Y Ruan M Li Q Wang J Zhu X Zhang, Li Cao, Zeng Zou, Yao Ruan, Min Li, Qinghua Wang, Jianxiang Zhu, Xiaofan affil: State Key Laboratory of Experimental Hematology, Department of Pediatrics, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, People's Republic of China sug: subj: Antineoplastic Agents Therapeutic Use Arsenicals Therapeutic Use Leukemia, Myeloid, Acute Diagnosis Leukemia, Myeloid, Acute Drug Therapy Proteins Oxides Therapeutic Use Tretinoin Therapeutic Use Adolescence Child Child, Preschool Prognosis Female Human Leukemia, Myeloid, Acute Male Multivariate Analysis Neoplastic Processes Polymerase Chain Reaction Disease Remission RNA Drug Effects Treatment Outcomes Adolescent: 13-18 years Child: 6-12 years Child, Preschool: 2-5 years Female Male ab: Reports on the use of the real-time quantitative polymerase chain reaction (RQ-PCR) for childhood acute promyelocytic leukemia (APL) therapy are scarce. This study describes the prognostic significance of quantification of the PML-RARa transcript in children with APL. Since January 2004, we have analyzed 40 children treated with all-trans-retinoic acid ± arsenic trioxide in induction. Thirty-nine patients (97.5%) entered complete remission. The 5-year rates of disease-free survival (DFS) and overall survival in these patients were 73.1 and 91.4%, respectively. By employing a standardized RQ-PCR protocol for minimal residual disease (MRD) monitoring, we determined that less than 1 normalized copy number (NCN) after induction indicates higher probability of a more favorable treatment outcome. After induction therapy, thirteen out of 38 (34.2%) patients in hematologic remission showed a negative RQ-PCR result (less than 1 NCN), which was correlated with the lower probability of relapse (100 and 55.2% DFS at 5 years in the negative and positive RQ-PCR groups, respectively; P = 0.018). PML/RARa-based MRD monitoring by RQ-PCR may allow us to identify subgroups of patients at low risk of relapse after induction. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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