In pulmonary sclerosing hemangioma expression of β-catenin, Axin, and C-myc differs between the two cell types.
Previous studies have shown that pulmonary sclerosing hemangioma is a tumor derived from primitive respiratory epithelium, but its character and the differentiation status of the two cell types (polygonal and cuboidal) composing the lesion are still controversial. We hypothesize that the polygonal c...
| Publicado en: | Virchows Archiv: European Journal of Pathology Vol. 461; no. 1; pp. 59 - 66 |
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| Autores principales: | , , , , , , , , , , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
Jul2012
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104444487&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104444487 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09456317 O1Z jtl: Virchows Archiv: European Journal of Pathology issn: 09456317 maglogo: N pubinfo: dt: Jul2012 vid: 461 iid: 1 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 104444487 NLM22614067 2011753451 10.1007/s00428-012-1247-6 NLM22614067 104444487 ppf: 59 ppct: 7 formats: fmt: @attributes: type: P tig: atl: In pulmonary sclerosing hemangioma expression of β-catenin, Axin, and C-myc differs between the two cell types. aug: au: Lin XY Zhang D Zhang Y Fan CF Dai SD Wang EH Lin, Xu-Yong Zhang, Di Zhang, Yong Fan, Chui-Feng Dai, Shun-Dong Wang, En-Hua affil: Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences, China Medical University, Shenyang 110001, China sug: subj: Proteins Lung Neoplasms Metabolism Lung Neoplasms Pathology Cytoskeletal Proteins Adult Aged Female Immunohistochemistry Specimen Handling Male Middle Age RNA Analysis Respiratory Mucosa Metabolism Respiratory Mucosa Pathology Reverse Transcriptase Polymerase Chain Reaction Adult: 19-44 years Aged: 65+ years Middle Aged: 45-64 years Female Male ab: Previous studies have shown that pulmonary sclerosing hemangioma is a tumor derived from primitive respiratory epithelium, but its character and the differentiation status of the two cell types (polygonal and cuboidal) composing the lesion are still controversial. We hypothesize that the polygonal cells are immature compared with cuboidal cells and have higher proliferative activity. To further study this question, we examined the expression of β-catenin, Axin, and C-myc by immunostaining in 45 primary sclerosing hemangioma (PSH) specimens. The two cell types were captured by laser capture microdissection from 28 PSH specimens, and total RNA was extracted. Messenger RNA (mRNA) expression of Axin and C-myc was examined by reverse transcription polymerase chain reaction (RT-PCR). By immunostaining, β-catenin was predominantly strongly expressed on the cell membrane of cuboidal cells, while in polygonal cells, β-catenin was predominantly expressed in the cytoplasm and significantly decreased on cell membranes. Axin was expressed in cuboidal cells in 93 % of our 45 cases, but only expressed in 18 % of these in polygonal cells. C-myc expression in polygonal cells was significantly stronger than in cuboidal cells (P < 0.05). RT-PCR showed that the expression level of Axin mRNA in cuboidal cells was significantly higher than in polygonal cells (P < 0.05), and expression level of C-myc mRNA in polygonal cells was significantly higher than in cuboidal cells (P < 0.05).The two PHS cell types have distinct expression of β-catenin, Axin, and C-myc, suggesting that their differentiation status may be different. The higher expression of C-myc in polygonal cells suggests that these cells might have higher proliferative activity. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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