Metallobeta-lactamase enzymes and antibiotic susceptibilities in strains of pseudomonas aeruginosa isolated from intensive care units in Turkey.

Objective: The aim of this study was to determine the frequency of metallobeta-lactamase (MBL) enzyme in Pseudomonas aeruginosa strains resistant to carbapenem (imipenem or/and meropenem) in seven regions of Turkey and to assess the minimal inhibitory concentration (MIC) levels of drugs used in trea...

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Publicado en:Turkiye Klinikleri Journal of Medical Sciences Vol. 32; no. 3; pp. 687 - 694
Autores principales: Cesur, Salih, Yildiz, Eda, Irmak, Hasan, Gülay, Zeynep, Arslan, Ugur, Nevgun, Sepin Özen, Bayramoglu, Gülçin, Berktas, Mustafa, Gencer, Serap, Hosoglu, Salih, Demiröz, Ali Pekcan
Formato: pictorial research tables/charts Journal Article
Publicado: Turkiye Klinikleri Jun2012
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Jun2012
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        atl: Metallobeta-lactamase enzymes and antibiotic susceptibilities in strains of pseudomonas aeruginosa isolated from intensive care units in Turkey.
      aug:
        au:
          Cesur, Salih
          Yildiz, Eda
          Irmak, Hasan
          Gülay, Zeynep
          Arslan, Ugur
          Nevgun, Sepin Özen
          Bayramoglu, Gülçin
          Berktas, Mustafa
          Gencer, Serap
          Hosoglu, Salih
          Demiröz, Ali Pekcan
      sug:
        subj:
          Drug Resistance, Microbial
          Intensive Care Units
          Microbial Culture and Sensitivity Tests
          Pseudomonas Infections Complications
          Antibiotics Adverse Effects
          Human
          In Vitro Studies
          Turkiye
      ab: Objective: The aim of this study was to determine the frequency of metallobeta-lactamase (MBL) enzyme in Pseudomonas aeruginosa strains resistant to carbapenem (imipenem or/and meropenem) in seven regions of Turkey and to assess the minimal inhibitory concentration (MIC) levels of drugs used in treatment such as colistin, aztreonam, polymyxin B and rifampin. Overall 186 Pseudomonas aeruginosa (P.aeruginosa) strains resistant to carbapenem from 8 provinces (Ankara, Konya, Antalya, I.stanbul, I.zmir, Diyarbak?r, Van and Trabzon) representing 7 different geographical regions of Turkey were included in the study. Material and Methods: The presence of MBL in P.aeruginosa strains resistant to carbapenem was investigated by combined disk methods with imipenem and EDTA absorbed imipenem disk. The MBL positivity was determined in the strains. Additionally, susceptibility to aztreonam, colistin, polymyxin B, and rifampin was established by the E-test method. Results: MBL enzyme positivity was detected in 58 out of 186 strains (31.2%). There was statistically significant difference between regions in terms of MBL positivity, with the highest rates in Antalya (52%), and I.stanbul (50%) and the lowest in Diyarbak?r (6%). Aztreonam sensitivity was detected in 134 (72 %) strains; 155 (83.3%) were sensitive to colistin and 148 (79.6%) to polymixine. No strain (0%) was sensitive to rifampin. Conclusion: In conclusion, the overall mean rate of MBL positivity was 31.2%, which is quite high. Therefore, it will be beneficial to confirm the MBL positivity of strains with molecular methods, to review regional antibiotic surveillance data at certain intervals and to share the obtained data with relevant institutions in order to prevent the regional spread of these strains. Thus, it is essential to record and monitor systematically the antibiotic surveillance data.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: Turkish
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