The effects of two different doses of dexmedetomidine in a testicular ischemia-reperfusion model in rats.
Objective: Dexmedetomidine is known to have anti-inflammatory activity in both ischemic and toxic inflammatory response models. In this study, the effects of two different doses of dexmedetomidine were investigated in a rat model of testicular ischemia-reperfusion. Material and Methods: Twenty eight...
| Published in: | Turkiye Klinikleri Journal of Medical Sciences Vol. 32; no. 3; pp. 766 - 775 |
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| Main Authors: | , , , , , , , |
| Format: | pictorial research tables/charts Journal Article |
| Published: |
Turkiye Klinikleri
Jun2012
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104446215&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104446215 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 13000292 1VOP jtl: Turkiye Klinikleri Journal of Medical Sciences issn: 13000292 maglogo: N pubinfo: dt: Jun2012 vid: 32 iid: 3 pid: 67298 pub: Turkiye Klinikleri artinfo: ui: 104446215 104446215 2011551594 10.5336/medsci.2011-26915 104446215 ppf: 766 ppct: 9 formats: fmt: @attributes: type: P tig: atl: The effects of two different doses of dexmedetomidine in a testicular ischemia-reperfusion model in rats. aug: au: Bektas, Hayri Özzeybek, Deniz Günenç, Fermim Akkus, Muhammed Bagriyanik, Alper Küme, Tuncay Aygün, HAkan Kizer, Onur sug: subj: Hypnotics and Sedatives Therapeutic Use Reperfusion Injury Drug Therapy Treatment Outcomes Animal Studies Malondialdehyde Blood Models, Biological Nitric Oxide Blood Rats Spermatic Cord Torsion Complications ab: Objective: Dexmedetomidine is known to have anti-inflammatory activity in both ischemic and toxic inflammatory response models. In this study, the effects of two different doses of dexmedetomidine were investigated in a rat model of testicular ischemia-reperfusion. Material and Methods: Twenty eight rats weighing 300-350 g were divided into four groups. The left testis and spermatical cord were liberated by left inguinal incision in all groups. Sham group (Group 1) (n=7) did not receive any other procedure. One hour of torsion and four hours of detorsion were applied to the other groups. The rats in ischemia/reperfusion group (Group 2) (I/R, n=7), did not receive any drug. Dexmedetomidine 50 µg/kg for Group 3 (I/R+50D, n=7) and 100 µg/kg for Group 4 (I/R+100D, n=7) was injected intraperitoneally after 30 mins of testicular torsion. To grade the testicular reperfusion injury tissue malondialdehyde (MDA) and nitric oxide (NO) levels were measured and the germ cell damage was evaluated histopathologically using Cosentino Scale in the excised testis. Results: MDA levels in group I/R were significantly higher than in sham, I/R+50D and I/R+100D groups (p<0.001). MDA levels in I/R+50D and I/R+100D were also significantly different (p=0.01). NO levels were significantly higher in group I/R when compared to Sham, I/R+50D and I/R+100D groups (p=0.003). Hystopathological scores were higher in I/R group than in I/R+50D and I/R+100D groups (p=0.001). Conclusion: Deksmedetomidine treatment at a dose of 50 and 100 µg/kg, attenuated reperfusion injury in a dose-dependent manner in this experimental testicular ischemia reperfusion model. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: Turkish refInfo: holdings: @attributes: islocal: N |
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