Comparative study of various E. coli strains for biohydrogen production applying response surface methodology.
The proper strategy to establish efficient hydrogen-producing biosystems is the biochemical, physiological characterization of hydrogen-producing microbes followed by metabolic engineering in order to give extraordinary properties to the strains and, finally, bioprocess optimization to realize enhan...
| Publicado en: | Scientific World Journal pp. 819793 - 819794 |
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| Autores principales: | , , |
| Formato: | research Journal Article |
| Publicado: |
Wiley-Blackwell
2012
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104456007&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104456007 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 1537744X 1BX5 jtl: Scientific World Journal issn: 1537744X maglogo: N pubinfo: dt: 2012 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 104456007 104456007 NLM22666156 2011572346 10.1100/2012/819793 NLM22666156 PMC3361317 104456007 ppf: 819793 ppct: 1 formats: tig: atl: Comparative study of various E. coli strains for biohydrogen production applying response surface methodology. aug: au: Bakonyi, Péter Nemestóthy, Nándor Bélafi-Bakó, Katalin affil: Research Institute on Bioengineering, Membrane Technology, and Energetics, University of Pannonia, Egyetem ut 10, 8200 Veszprém, Hungary. sug: subj: Escherichia Coli Metabolism Hydrogen Metabolism Fermentation Surface Properties ab: The proper strategy to establish efficient hydrogen-producing biosystems is the biochemical, physiological characterization of hydrogen-producing microbes followed by metabolic engineering in order to give extraordinary properties to the strains and, finally, bioprocess optimization to realize enhanced hydrogen fermentation capability. In present paper, it was aimed to show the utility both of strain engineering and process optimization through a comparative study of wild-type and genetically modified E. coli strains, where the effect of two major operational factors (substrate concentration and pH) on bioH₂ production was investigated by experimental design and response surface methodology (RSM) was used to determine the suitable conditions in order to obtain maximum yields. The results revealed that by employing the genetically engineered E. coli (DJT 135) strain under optimized conditions (pH: 6.5; Formate conc.: 1.25 g/L), 0.63 mol H₂/mol formate could be attained, which was 1.5 times higher compared to the wild-type E. coli (XL1-BLUE) that produced 0.42 mol H₂/mol formate (pH: 6.4; Formate conc.: 1.3 g/L). pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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