Exposure to Ambient Air Fine Particulate Matter Prevents VEGF-Induced Mobilization of Endothelial Progenitor Cells from the Bone Marrow.
Background: Exposure to ambient fine particulate matter air pollution (PM2.5; < 2.5 μm in aerodynamic diameter) induces endothelial dysfunction and increases the risk for cardiovascular disease. Endothelial progenitor cells (EPCs) contribute to postnatal endothelial repair and regeneration. In human...
| Publicado en: | Environmental Health Perspectives Vol. 120; no. 6; pp. 848 - 857 |
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| Autores principales: | , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
National Institute of Environmental Health Sciences
Jun2012
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104461997&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104461997 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00916765 3B5 jtl: Environmental Health Perspectives issn: 00916765 maglogo: N pubinfo: dt: Jun2012 vid: 120 iid: 6 pid: 56539 pub: National Institute of Environmental Health Sciences place: Research Triangle Park, North Carolina artinfo: ui: 104461997 76451518 10.1289/ehp.1104206 NLM22418586 104461997 ppf: 848 ppct: 9 formats: fmt: @attributes: type: P tig: atl: Exposure to Ambient Air Fine Particulate Matter Prevents VEGF-Induced Mobilization of Endothelial Progenitor Cells from the Bone Marrow. aug: au: Haberzettl, Petra Jongmin Lee Duggineni, Dheeraj McCracken, James Bolanowski, Duane O¿Toole, Timothy E. Bhatnagar, Aruni Conklin, Daniel J. affil: Diabetes and Obesity Center, University of Louisville, Louisville, Kentucky, USA sug: subj: Particulate Matter Adverse Effects Air Pollution Adverse Effects Endothelium Drug Effects Bone Marrow Drug Effects Vascular Endothelial Growth Factors Drug Effects Environmental Exposure Adverse Effects Cardiovascular Risk Factors Mice Animal Studies Kentucky Vascular Endothelial Growth Factors Administration and Dosage Flow Cytometry Blotting, Western Cell Culture Techniques Descriptive Statistics T-Tests Post Hoc Analysis One-Way Analysis of Variance Funding Source ab: Background: Exposure to ambient fine particulate matter air pollution (PM2.5; < 2.5 μm in aerodynamic diameter) induces endothelial dysfunction and increases the risk for cardiovascular disease. Endothelial progenitor cells (EPCs) contribute to postnatal endothelial repair and regeneration. In humans and mice, EPC levels are decreased upon exposure to elevated levels of PM2.5.Objective: We examined the mechanism by which PM2.5 exposure suppresses circulating levels of EPCs.Methods: Mice were exposed to HEPA-filtered air or concentrated ambient fine particulate matter (CAP, 30–100 μg/m3) from downtown Louisville (Kentucky) air, and progenitor cells from peripheral blood or bone marrow were analyzed by flow cytometry or by culture ex vivo.Results: Exposure of the mice to CAP (6 hr/day) for 4–30 days progressively decreased circulating levels of EPCs positive for both Flk‑1 and Sca‑1 (Flk‑1+/Sca‑1+) without affecting stem cells positive for Sca‑1 alone (Sca‑1+). After 9 days of exposure, a 7‑day exposure-free period led to complete recovery of the circulating levels of Flk‑1+/Sca‑1+ cells. CAP exposure decreased circulating levels of EPCs independent of apoptosis while simultaneously increasing Flk‑1+/Sca‑1+ cells in the bone marrow. We observed no change in tissue deposition of these cells. CAP exposure suppressed vascular endothelial growth factor (VEGF)-induced Akt and endothelial nitric oxide synthase (eNOS) phosphorylation in the aorta, and it prevented VEGF/AMD3100-induced mobilization of Flk‑1+/Sca‑1+ cells into the peripheral blood. Treatment with stem cell factor/AMD3100 led to a greater increase in circulating Flk‑1+/Sca‑1+ cells in CAP-exposed mice than in mice breathing filtered air. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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