Loss of cellular viability in areas of ground-glass opacity on computed tomography images immediately after pulmonary radiofrequency ablation in rabbits.

Purpose: To determine cellular viability of lung parenchyma and neoplastic cells in areas of ground-glass opacity (GGO) on computed tomography (CT) images immediately after pulmonary radiofrequency ablation (RFA) in rabbits.Materials and Methods: A LeVeen RFA electrode was placed percutaneously into...

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Publicado en:Japanese Journal of Radiology Vol. 30; no. 4; pp. 323 - 331
Autores principales: Kuroki M, Nakada H, Yamashita A, Sawaguchi A, Uchino N, Sato S, Asanuma T, Asada Y, Tamura S, Kuroki, Masaomi, Nakada, Hiroshi, Yamashita, Atsushi, Sawaguchi, Akira, Uchino, Noriko, Sato, Shinya, Asanuma, Taketoshi, Asada, Yujiro, Tamura, Shozo
Formato: research Journal Article
Publicado: Springer Nature May2012
Acceso en línea:Ver este registro en EBSCOhost
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      dt: May2012
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      pub: Springer Nature
      place: New York, New York
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        atl: Loss of cellular viability in areas of ground-glass opacity on computed tomography images immediately after pulmonary radiofrequency ablation in rabbits.
      aug:
        au:
          Kuroki M
          Nakada H
          Yamashita A
          Sawaguchi A
          Uchino N
          Sato S
          Asanuma T
          Asada Y
          Tamura S
          Kuroki, Masaomi
          Nakada, Hiroshi
          Yamashita, Atsushi
          Sawaguchi, Akira
          Uchino, Noriko
          Sato, Shinya
          Asanuma, Taketoshi
          Asada, Yujiro
          Tamura, Shozo
        affil: Department of Radiology, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan
      sug:
        subj:
          Catheter Ablation Methods
          Lung Neoplasms Radiography
          Lung Neoplasms Surgery
          Tomography, X-Ray Computed
          Analysis of Variance
          Animals
          Catheter Ablation Equipment and Supplies
          Cell Physiology
          Equipment Design
          Fluoroscopy
          Lung Neoplasms Pathology
          Prospective Studies
          Rabbits
          Staining and Labeling
      ab: Purpose: To determine cellular viability of lung parenchyma and neoplastic cells in areas of ground-glass opacity (GGO) on computed tomography (CT) images immediately after pulmonary radiofrequency ablation (RFA) in rabbits.Materials and Methods: A LeVeen RFA electrode was placed percutaneously into rabbit lungs with or without metastatic VX2 tumors. Five minutes later, seven isolated lungs were imaged by use of a multi-detector row CT scanner, and the images were compared with histological features. The cellular viability of the lung tissues was assessed by nicotinamide adenine dinucleotide hydrogen (NADH) staining in eight normal lungs and in three lungs with multiple metastatic tumors.Results: All lung lesions appeared as bilayered structures with a central, dense, attenuated area and an outer area of GGO on CT images, and as three-layered structures on macroscopic and microscopic images 5 min after RFA. The GGO areas approximately corresponded to the outer two layers in macroscopic images that were exudative and congestive on microscopic images. Staining for NADH was significantly reduced in the GGO and densely attenuated areas with or without tumor tissue staining compared with the non-ablated area.Conclusions: Our results suggest that an area of GGO that appears on CT immediately after RFA can be effectively treated by RFA.
      pubtype: Academic Journal
      doctype:
        research
        Journal Article
      ougenre: Article
    language: English
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