Quercetin regulates organic ion transporter and uromodulin expression and improves renal function in hyperuricemic mice.
Background: Renal organic ion transporters and uromodulin (UMOD) play the important roles in renal urate excretion and function. Hyperuricemia is considered as a risk factor for the development of renal dysfunction. The flavonoid quercetin in diets exerts the hypouricemic and nephroprotective effect...
| Publicado en: | European Journal of Nutrition Vol. 51; no. 5; pp. 593 - 607 |
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| Autores principales: | , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Springer Nature
Aug2012
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104477051&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104477051 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 14366207 CR0 jtl: European Journal of Nutrition issn: 14366207 maglogo: N pubinfo: dt: Aug2012 vid: 51 iid: 5 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 104477051 77735110 10.1007/s00394-011-0243-y NLM21909718 104477051 ppf: 593 ppct: 14 formats: fmt: @attributes: type: P tig: atl: Quercetin regulates organic ion transporter and uromodulin expression and improves renal function in hyperuricemic mice. aug: au: Hu, Qing-Hua Zhang, Xian Wang, Xing Jiao, Rui-Qing Kong, Ling-Dong affil: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093 People's Republic of China sug: subj: Uric Acid Metabolism Kidney Drug Effects Quercetin Pharmacodynamics Symporters Drug Effects Membrane Glycoproteins Drug Effects China Funding Source Animal Studies In Vivo Studies Mice Comparative Studies Dose-Response Relationship, Drug Uric Acid Blood Creatinine Blood Blood Urea Nitrogen Evaluation Urinalysis Histological Techniques Nephrotoxicity Prevention and Control Reverse Transcriptase Polymerase Chain Reaction Blotting, Western Descriptive Statistics Two-Way Analysis of Variance Post Hoc Analysis ab: Background: Renal organic ion transporters and uromodulin (UMOD) play the important roles in renal urate excretion and function. Hyperuricemia is considered as a risk factor for the development of renal dysfunction. The flavonoid quercetin in diets exerts the hypouricemic and nephroprotective effects. Purposes: To evaluate the effects of quercetin on renal organic ion transporters and UMOD in hyperuricemic mice. Methods: Kun-Ming mice were divided into normal and hyperuricemic groups receiving water, 25, 50 and 100 mg/kg quercetin, 5 mg/kg allopurinol, respectively. Hyperuricemic mice were orally gavaged with 250 mg/kg oxonate daily for 1 week. Quercetin and allopurinol were orally gavaged on the day when oxonate or water was given 1 h later. After 1 week, serum uric acid, creatinine and blood urea nitrogen concentrations, excretion of urate and creatinine, and fractional excretion of uric acid were measured. The mRNA and protein levels of renal urate transporter 1 (mURAT1), glucose transporter 9 (mGLUT9), organic anion transporter 1 (mOAT1) and organic cation/carnitine transporters (mOCT1, mOCT2, mOCTN1 and mOCTN2) in mice were analyzed. Simultaneously, UMOD levels in serum, urine and kidney, as well as renal UMOD mRNA expression were detected. Results: Quercetin significantly restored oxonate-induced abnormalities of these biochemical indexes compared with normal vehicle group. Furthermore, it remarkably prevented expression changes of renal organic ion transporters and UMOD, and UMOD level alteration in hyperuricemic mice. Conclusions: These results suggest that quercetin has the uricosuric and nephroprotective actions mediated by regulating the expression levels of renal organic ion transporters and UMOD. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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