Biological and clinical significance of p75NTR expression in laryngeal squamous epithelia and laryngocarcinoma.

Conclusion: The apparent features of p75 neurotrophin receptor (p75NTR) expression indicated that p75NTR would serve as a potential stem cell marker for normal human laryngeal squamous epithelia. In human laryngeal squamous cell carcinoma (LSCC) p75NTR is differentially expressed. The abnormal expre...

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Publicado en:Acta Oto-Laryngologica Vol. 132; no. 3; pp. 314 - 325
Autores principales: Li, Xiaoming, Shen, Yupeng, Di, Bin, Li, Jun, Geng, Jiangqiao, Lu, Xiuying, He, Zhanguo
Formato: pictorial research tables/charts Journal Article
Publicado: Taylor & Francis Ltd Mar2012
Acceso en línea:Ver este registro en EBSCOhost
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      pub: Taylor & Francis Ltd
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        atl: Biological and clinical significance of p75NTR expression in laryngeal squamous epithelia and laryngocarcinoma.
      aug:
        au:
          Li, Xiaoming
          Shen, Yupeng
          Di, Bin
          Li, Jun
          Geng, Jiangqiao
          Lu, Xiuying
          He, Zhanguo
      sug:
        subj:
          Carcinoma, Squamous Cell Diagnosis
          Laryngeal Diseases Diagnosis
          Receptors, Cell Surface Diagnostic Use
          Receptors, Cell Surface Blood
          Human
          Male
          Female
          Immunohistochemistry Methods
          Flow Cytometry
          Stem Cells Microbiology
          Tumor Markers, Biological
          Data Analysis Software
          Chi Square Test
          Nerve Growth Factors
          Spearman's Rank Correlation Coefficient
          Wilcoxon Signed Rank Test
          Adult
          Middle Age
          Aged
          Funding Source
          Adult: 19-44 years
          Middle Aged: 45-64 years
          Aged: 65+ years
          Male
          Female
      ab: Conclusion: The apparent features of p75 neurotrophin receptor (p75NTR) expression indicated that p75NTR would serve as a potential stem cell marker for normal human laryngeal squamous epithelia. In human laryngeal squamous cell carcinoma (LSCC) p75NTR is differentially expressed. The abnormal expression and distribution of p75NTR may indicate malignant transformation. Objective: To investigate the expression of p75NTR and its possible roles in normal laryngeal squamous epithelia and LSCC. Methods: We used immunohistochemistry methods to examine normal laryngeal epithelia, para-cancer mucosa with dysplasia, laryngeal papilloma, and LSCC specimens for the expression of p75NTR, nerve growth factor (NGF), -tyrosine kinase receptor (TrkA), p63, and Ki67. Immunocytochemistry and flow cytometry were used to examine the expression of p75NTR in Hep-2 cells. Results: The expression of p75NTR was only located in basal cells of normal laryngeal epithelia, consistent with the staining features of epithelial stem cells as evidenced by parallel staining of p63, a putative keratinocyte stem cell marker. p75NTR is differentially expressed in LSCC, although no significant relationship was found with many clinicopathologic factors, this expression and distribution may correlate to malignant transformation and tumor proliferation. Co-expression of p75NTR and CD133 was confirmed, showing the association of p75NTR-positive cells with cancer stem cells in Hep-2 cells.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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