A Comparative BAC map for the gilthead sea bream (Sparus aurata L.)
This study presents the first comparative BAC map of the gilthead sea bream (Sparus aurata), a highly valuated marine aquaculture fish species in the Mediterranean. High-throughput end sequencing of a BAC library yielded 92,468 reads (60.6 Mbp). Comparative mapping was achieved by anchoring BAC end...
| Publicado en: | Journal of Biomedicine & Biotechnology pp. 329025 - 329026 |
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| Autores principales: | , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Wiley-Blackwell
2011
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104531456&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104531456 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 11107243 137K jtl: Journal of Biomedicine & Biotechnology issn: 11107243 maglogo: N pubinfo: dt: 2011 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 104531456 104531456 2011456867 NLM21049003 PMC2964914 104531456 ppf: 329025 ppct: 1 formats: fmt: @attributes: type: P tig: atl: A Comparative BAC map for the gilthead sea bream (Sparus aurata L.) aug: au: Kuhl, Heiner Sarropoulou, Elena Tine, Mbaye Kotoulas, Georgios Magoulas, Antonios Reinhardt, Richard affil: Max Planck Institute for Molecular Genetics, htpt group, Ihnestr. 63, 14195 Berlin, Germany. kuhl@molgen.mpg.de sug: subj: Chromosomes Fish Animals Chromosome Mapping Methods Genetic Techniques Sequence Analysis Methods ab: This study presents the first comparative BAC map of the gilthead sea bream (Sparus aurata), a highly valuated marine aquaculture fish species in the Mediterranean. High-throughput end sequencing of a BAC library yielded 92,468 reads (60.6 Mbp). Comparative mapping was achieved by anchoring BAC end sequences to the three-spined stickleback (Gasterosteus aculeatus) genome. BACs that were consistently ordered along the stickleback chromosomes accounted for 14,265 clones. A fraction of 5,249 BACs constituted a minimal tiling path that covers 73.5% of the stickleback chromosomes and 70.2% of the genes that have been annotated. The N50 size of 1,485 'BACtigs' consisting of redundant BACs is 337,253 bp. The largest BACtig covers 2.15 Mbp in the stickleback genome. According to the insert size distribution of mapped BACs the sea bream genome is 1.71-fold larger than the stickleback genome. These results represent a valuable tool to researchers in the field and may support future projects to elucidate the whole sea bream genome. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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