BAC libraries from wheat chromosome 7D: efficient tool for positional cloning of aphid resistance genes.
Positional cloning in bread wheat is a tedious task due to its huge genome size and hexaploid character. BAC libraries represent an essential tool for positional cloning. However, wheat BAC libraries comprise more than million clones, which makes their screening very laborious. Here, we present a ta...
| Publicado en: | Journal of Biomedicine & Biotechnology pp. 302543 - 302544 |
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| Autores principales: | , , , , , , , , , , , , , , |
| Formato: | Journal Article |
| Publicado: |
Wiley-Blackwell
2011
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104531542&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104531542 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 11107243 137K jtl: Journal of Biomedicine & Biotechnology issn: 11107243 maglogo: N pubinfo: dt: 2011 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 104531542 2011456953 NLM21318113 PMC3035010 104531542 ppf: 302543 ppct: 1 formats: fmt: @attributes: type: P tig: atl: BAC libraries from wheat chromosome 7D: efficient tool for positional cloning of aphid resistance genes. aug: au: Simková, Hana Safá, Jan Kubaláková, Marie Suchánková, Pavla Cíhalíková, Jarmila Robert-Quatre, Heda Azhaguvel, Perumal Weng, Yiqun Peng, Junhua Lapitan, Nora L V Ma, Yaqin You, Frank M Luo, Ming-Cheng Bartos, Jan Dolezel, Jaroslav affil: Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Sokolovská 6, CZ 772 00 Olomouc, Czech Republic. simkovah@ueb.cas.cz sug: subj: Insects Physiology Chromosomes Genetic Techniques Methods Immunity Plant Diseases Immunology Wheat Animals Light Genes Karyotyping DNA Nucleic Acid Hybridization Plant Diseases Polymerase Chain Reaction ab: Positional cloning in bread wheat is a tedious task due to its huge genome size and hexaploid character. BAC libraries represent an essential tool for positional cloning. However, wheat BAC libraries comprise more than million clones, which makes their screening very laborious. Here, we present a targeted approach based on chromosome-specific BAC libraries. Such libraries were constructed from flow-sorted arms of wheat chromosome 7D. A library from the short arm (7DS) consisting of 49,152 clones with 113 kb insert size represented 12.1 arm equivalents whereas a library from the long arm (7DL) comprised 50,304 clones of 116 kb providing 14.9x arm coverage. The 7DS library was PCR screened with markers linked to Russian wheat aphid resistance gene DnCI2401, the 7DL library was screened by hybridization with a probe linked to greenbug resistance gene Gb3. The small number of clones combined with high coverage made the screening highly efficient and cost effective. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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