Prediction of B-cell linear epitopes with a combination of support vector machine classification and amino acid propensity identification.

Epitopes are antigenic determinants that are useful because they induce B-cell antibody production and stimulate T-cell activation. Bioinformatics can enable rapid, efficient prediction of potential epitopes. Here, we designed a novel B-cell linear epitope prediction system called LEPS, Linear Epito...

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Publicado en:Journal of Biomedicine & Biotechnology pp. 432830 - 432831
Autores principales: Wang, Hsin-Wei, Lin, Ya-Chi, Pai, Tun-Wen, Chang, Hao-Teng
Formato: Journal Article
Publicado: Wiley-Blackwell 2011
Acceso en línea:Ver este registro en EBSCOhost
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      pub: Wiley-Blackwell
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        atl: Prediction of B-cell linear epitopes with a combination of support vector machine classification and amino acid propensity identification.
      aug:
        au:
          Wang, Hsin-Wei
          Lin, Ya-Chi
          Pai, Tun-Wen
          Chang, Hao-Teng
        affil: Department of Computer Science and Engineering, National Taiwan Ocean University, Keelung, Taiwan.
      sug:
        subj:
          Bioinformatics Methods
          Resource Databases
          Antigens Analysis
          Algorithms
          Amino Acids
          Antigens
          Models, Theoretical
          Models, Statistical
          User-Computer Interface
      ab: Epitopes are antigenic determinants that are useful because they induce B-cell antibody production and stimulate T-cell activation. Bioinformatics can enable rapid, efficient prediction of potential epitopes. Here, we designed a novel B-cell linear epitope prediction system called LEPS, Linear Epitope Prediction by Propensities and Support Vector Machine, that combined physico-chemical propensity identification and support vector machine (SVM) classification. We tested the LEPS on four datasets: AntiJen, HIV, a newly generated PC, and AHP, a combination of these three datasets. Peptides with globally or locally high physicochemical propensities were first identified as primitive linear epitope (LE) candidates. Then, candidates were classified with the SVM based on the unique features of amino acid segments. This reduced the number of predicted epitopes and enhanced the positive prediction value (PPV). Compared to four other well-known LE prediction systems, the LEPS achieved the highest accuracy (72.52%), specificity (84.22%), PPV (32.07%), and Matthews' correlation coefficient (10.36%).
      pubtype: Academic Journal
      doctype: Journal Article
      ougenre: Article
    language: English
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